A single-stranded architecture for cotranscriptional folding of RNA nanostructures - PubMed (original) (raw)
. 2014 Aug 15;345(6198):799-804.
doi: 10.1126/science.1253920.
Affiliations
- PMID: 25124436
- DOI: 10.1126/science.1253920
A single-stranded architecture for cotranscriptional folding of RNA nanostructures
Cody Geary et al. Science. 2014.
Abstract
Artificial DNA and RNA structures have been used as scaffolds for a variety of nanoscale devices. In comparison to DNA structures, RNA structures have been limited in size, but they also have advantages: RNA can fold during transcription and thus can be genetically encoded and expressed in cells. We introduce an architecture for designing artificial RNA structures that fold from a single strand, in which arrays of antiparallel RNA helices are precisely organized by RNA tertiary motifs and a new type of crossover pattern. We constructed RNA tiles that assemble into hexagonal lattices and demonstrated that lattices can be made by annealing and/or cotranscriptional folding. Tiles can be scaled up to 660 nucleotides in length, reaching a size comparable to that of large natural ribozymes.
Copyright © 2014, American Association for the Advancement of Science.
Comment in
- Self-assembled RNA nanostructures.
Leontis NB, Westhof E. Leontis NB, et al. Science. 2014 Aug 15;345(6198):732-3. doi: 10.1126/science.1257989. Science. 2014. PMID: 25124412 No abstract available.
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