The mechanism of guanosine nucleotide hydrolysis by p21 c-Ha-ras. The stereochemical course of the GTPase reaction - PubMed (original) (raw)
. 1989 Apr 15;264(11):6188-90.
Affiliations
- PMID: 2539374
Free article
The mechanism of guanosine nucleotide hydrolysis by p21 c-Ha-ras. The stereochemical course of the GTPase reaction
J Feuerstein et al. J Biol Chem. 1989.
Free article
Abstract
The use of guanosine 5'-O-(gamma-thio)triphosphate as a substrate for p21 c-Ha-ras was established. By using chirally labeled [gamma-17O,18O]guanosine 5'-O-(gamma-thio)triphosphate, the stereochemical course of the GTPase reaction was determined. The analysis shows that the hydrolysis occurs with inversion at the gamma-phosphorus. This shows that the most likely mechanism is a single step, in-line transfer, without a phosphoenzyme or other phosphorylated intermediate.
Similar articles
- The stereochemical course of the ribosome-dependent GTPase reaction of elongation factor G from Escherichia coli.
Webb MR, Eccleston JF. Webb MR, et al. J Biol Chem. 1981 Aug 10;256(15):7734-7. J Biol Chem. 1981. PMID: 6114949 - [Possible mechanisms of disorders of GTPase activity with substitutions at the 12th and 61st codons of the p21 H-ras protein].
Lalaiants IE. Lalaiants IE. Vopr Onkol. 1987;33(7):9-15. Vopr Onkol. 1987. PMID: 3039739 Review. Russian. No abstract available.
Cited by
- The biochemistry of ras p21.
Grand RJ, Owen D. Grand RJ, et al. Biochem J. 1991 Nov 1;279 ( Pt 3)(Pt 3):609-31. doi: 10.1042/bj2790609. Biochem J. 1991. PMID: 1953656 Free PMC article. Review. No abstract available. - Monitoring the GAP catalyzed H-Ras GTPase reaction at atomic resolution in real time.
Allin C, Ahmadian MR, Wittinghofer A, Gerwert K. Allin C, et al. Proc Natl Acad Sci U S A. 2001 Jul 3;98(14):7754-9. doi: 10.1073/pnas.131549798. Proc Natl Acad Sci U S A. 2001. PMID: 11438727 Free PMC article. - X-ray crystal structures of transforming p21 ras mutants suggest a transition-state stabilization mechanism for GTP hydrolysis.
Privé GG, Milburn MV, Tong L, de Vos AM, Yamaizumi Z, Nishimura S, Kim SH. Privé GG, et al. Proc Natl Acad Sci U S A. 1992 Apr 15;89(8):3649-53. doi: 10.1073/pnas.89.8.3649. Proc Natl Acad Sci U S A. 1992. PMID: 1565661 Free PMC article. - Kinetic characterization of guanine-nucleotide-induced exocytosis from permeabilized rat mast cells.
Lillie TH, Gomperts BD. Lillie TH, et al. Biochem J. 1993 Mar 1;290 ( Pt 2)(Pt 2):389-94. doi: 10.1042/bj2900389. Biochem J. 1993. PMID: 8452525 Free PMC article. - Interactions of the ras-like protein p25rab3A with Mg2+ and guanine nucleotides.
Burstein ES, Macara IG. Burstein ES, et al. Biochem J. 1992 Mar 1;282 ( Pt 2)(Pt 2):387-92. doi: 10.1042/bj2820387. Biochem J. 1992. PMID: 1312327 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials