Transcriptomic correlates of organ failure extent in sepsis - PubMed (original) (raw)

doi: 10.1016/j.jinf.2014.12.010. Epub 2014 Dec 31.

María Heredia-Rodríguez 2, Esther Gomez-Sanchez 3, David Andaluz-Ojeda 4, Verónica Iglesias 5, Lucia Rico 6, Alicia Ortega 7, Estefanía Gomez-Pesquera 8, Pilar Liu 9, Marta Aragón 10, Jose Maria Eiros 11, Maria Ángeles Jiménez-Sousa 12, Salvador Resino 13, Ignacio Gómez-Herreras 14, Jesús F Bermejo-Martín 15, Eduardo Tamayo 16

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Transcriptomic correlates of organ failure extent in sepsis

Raquel Almansa et al. J Infect. 2015 May.

Abstract

Objectives: Sepsis is characterised by the frequent presence of organ failure and marked immunologic alterations. We studied the association between the extent of organ failure and the transcriptomic response of septic patients.

Methods: Gene expression profiles in the blood of 74 surgical patients with sepsis were compared with those of 30 surgical patients with no sepsis. Differentially expressed genes were assessed for their correlation with the sequential organ failure (SOFA) score.

Results: The expression levels of a group of genes participating in the cell cycle (HIST1H1C, CKS2, CCNA2, CDK1, CCNB2, CIT, CCNB1, AURKA, RAD51), neutrophil protease activity (ELANE, ADORA3, MPO, MMP8, CTSG), IL-1R and IL-18R response correlated directly with SOFA and mortality. Genes involved in T cell (LCK, CD3G, CD3D, ZAP70, ICOS, CD3E, CD28, IL2RB, CD8B, CD8A, CD40LG, IL23A, CCL5, SH2D1A, ITK, CD247, TBX21, GATA3, CCR7, LEF1, STAT4) and NK cell immunity (CD244, KLRK1, KLRD1) were inversely associated with SOFA and mortality.

Conclusions: The extent of organ failure in sepsis correlates directly with the existence of imbalanced innate and adaptive responses at the transcriptomic level. Quantification of the expression levels of the genes identified here could contribute to the simultaneous assessment of disease severity and immunological alterations in sepsis.

Keywords: Immune response; Immunosupression; Microarrays; Organ failure extent; SOFA; Sepsis; Transcriptomics.

Copyright © 2014 The British Infection Association. Published by Elsevier Ltd. All rights reserved.

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