Immunohistochemical Expressions of MUC2, MUC5AC, and MUC6 in Normal, Helicobacter pylori Infected and Metaplastic Gastric Mucosa of Children and Adolescents - PubMed (original) (raw)
Observational Study
doi: 10.1111/hel.12198. Epub 2015 Feb 23.
Jung-Sook Yeom 1, Ji-Hyun Seo 1, Jae-Young Lim 1, Chan-Hoo Park 1, Hyang-Ok Woo 1, Hee-Shang Youn 1, Jin-Su Jun 1, Ji-Hoe Park 1, Gyung-Hyuck Ko 2, Seung-Chul Baik 3, Woo-Kon Lee 3, Myung-Je Cho 3, Kwang-Ho Rhee 3
Affiliations
- PMID: 25704078
- DOI: 10.1111/hel.12198
Observational Study
Immunohistochemical Expressions of MUC2, MUC5AC, and MUC6 in Normal, Helicobacter pylori Infected and Metaplastic Gastric Mucosa of Children and Adolescents
Ji Sook Park et al. Helicobacter. 2015 Aug.
Abstract
Objectives: The aim of this study was to investigate expression of gastric mucins in children and adolescents and to assess their relations with age and Helicobacter pylori (H. pylori) infection.
Methods: Gastric biopsies were collected from 259 pediatric and adulthood patients with gastrointestinal symptoms among all of patients undergone gastroduodenoscopy from 1990 to 2004 at Gyeongsang National University hospital and assorted based on H. pylori infection, age, and intestinal metaplasia as follows; H. pylori infection before 5 years of age or not, H. pylori infection between 5 and 9 years of age or not, H. pylori infection between 10 and 14 years of age or not, H. pylori infection between 20 and 29 years of age or not and intestinal metaplasia between 21 and 35 years of age. Total 810 tissue slides from the subjects were examined regarding expressions of Mucin2 (MUC2), Mucin5AC (MUC5AC), and Mucin6 (MUC6) in nine groups using immunohistochemical stains. A semiquantitative approach was used to score the staining extent of tissue slide.
Results: Increased expressions of MUC2, MUC5AC, and MUC6 were noted in intestinal metaplasia compared with subjects infected with H. pylori between 20 and 29 years. Gastric expressions of MUC5AC were decreased in older than 5 years with H. pylori compared with in older than 5 years without H. pylori (p < .001). Expressions of MUC2 and MUC6 did not change significantly by H. pylori status. Some nuclear expressions of MUC2 and MUC6 were noted in children without intestinal metaplasia.
Conclusions: MUC5AC might be affected by chronic H. pylori infection. In addition to biomarkers for intestinal metaplasia or prognostic factors for gastric cancer in adults, MUC2 and MUC6 in children might have an another role, based on ectopic gastric nuclear expressions of MUC2 and MUC6 in children without intestinal metaplasia.
Keywords: Helicobacter pylori; Immunohistochemistry; gastric mucin.
© 2015 John Wiley & Sons Ltd.
Similar articles
- Immunohistochemical evaluation of mucin expression in precancerous tissue of stomach.
Vernygorodskyi S. Vernygorodskyi S. Exp Oncol. 2013 Jun;35(2):114-7. Exp Oncol. 2013. PMID: 23828387 - Effects of Helicobacter pylori Infection on gastric mucin expression.
Kang HM, Kim N, Park YS, Hwang JH, Kim JW, Jeong SH, Lee DH, Lee HS, Jung HC, Song IS. Kang HM, et al. J Clin Gastroenterol. 2008 Jan;42(1):29-35. doi: 10.1097/MCG.0b013e3180653cb7. J Clin Gastroenterol. 2008. PMID: 18097286 - Expression profile of mucins (MUC2, MUC5AC and MUC6) in Helicobacter pylori infected pre-neoplastic and neoplastic human gastric epithelium.
Babu SD, Jayanthi V, Devaraj N, Reis CA, Devaraj H. Babu SD, et al. Mol Cancer. 2006 Mar 19;5:10. doi: 10.1186/1476-4598-5-10. Mol Cancer. 2006. PMID: 16545139 Free PMC article. - Helicobacter pylori and gastric mucin expression: A systematic review and meta-analysis.
Niv Y. Niv Y. World J Gastroenterol. 2015 Aug 21;21(31):9430-6. doi: 10.3748/wjg.v21.i31.9430. World J Gastroenterol. 2015. PMID: 26309370 Free PMC article. Review. - [The role of gastric mucins in interactions with Helicobacter pylori].
Radziejewska I. Radziejewska I. Postepy Hig Med Dosw (Online). 2012 Jan 30;66:60-6. Postepy Hig Med Dosw (Online). 2012. PMID: 22371407 Review. Polish.
Cited by
- Upregulation of MUC5AC production and deposition of LEWIS determinants by HELICOBACTER PYLORI facilitate gastric tissue colonization and the maintenance of infection.
Gonciarz W, Walencka M, Moran AP, Hinc K, Obuchowski M, Chmiela M. Gonciarz W, et al. J Biomed Sci. 2019 Mar 6;26(1):23. doi: 10.1186/s12929-019-0515-z. J Biomed Sci. 2019. PMID: 30841890 Free PMC article. - Sera Antibody Repertoire Analyses Reveal Mechanisms of Broad and Pandemic Strain Neutralizing Responses after Human Norovirus Vaccination.
Lindesmith LC, McDaniel JR, Changela A, Verardi R, Kerr SA, Costantini V, Brewer-Jensen PD, Mallory ML, Voss WN, Boutz DR, Blazeck JJ, Ippolito GC, Vinje J, Kwong PD, Georgiou G, Baric RS. Lindesmith LC, et al. Immunity. 2019 Jun 18;50(6):1530-1541.e8. doi: 10.1016/j.immuni.2019.05.007. Immunity. 2019. PMID: 31216462 Free PMC article. - Idiopathic Pulmonary Fibrosis: A Genetic Disease That Involves Mucociliary Dysfunction of the Peripheral Airways.
Evans CM, Fingerlin TE, Schwarz MI, Lynch D, Kurche J, Warg L, Yang IV, Schwartz DA. Evans CM, et al. Physiol Rev. 2016 Oct;96(4):1567-91. doi: 10.1152/physrev.00004.2016. Physiol Rev. 2016. PMID: 27630174 Free PMC article. Review. - BabA-mediated adherence of pediatric ulcerogenic H. pylori strains to gastric mucins at neutral and acidic pH.
Quintana-Hayashi MP, Rocha R, Padra M, Thorell A, Jin C, Karlsson NG, Roxo-Rosa M, Oleastro M, Lindén SK. Quintana-Hayashi MP, et al. Virulence. 2018;9(1):1699-1717. doi: 10.1080/21505594.2018.1532243. Virulence. 2018. PMID: 30298790 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Medical
Miscellaneous