Effect of dietary supplementation of Bacillus subtilis B10 on biochemical and molecular parameters in the serum and liver of high-fat diet-induced obese mice - PubMed (original) (raw)

Effect of dietary supplementation of Bacillus subtilis B10 on biochemical and molecular parameters in the serum and liver of high-fat diet-induced obese mice

Kai Lei et al. J Zhejiang Univ Sci B. 2015 Jun.

Abstract

While a high-fat diet (HFD) is assumed to be related to fat-mediated oxidative stress decreasing antioxidant enzyme activity, probiotics are believed to have positive effects on the regulation of HFD-induced obesity as well as lipid metabolism, energy homeostasis, and anti-oxidation. Because Bacillus subtilis B10 has beneficial effects on the abnormal lipid metabolism and the oxidative stress in HFD-induced obese mice, ICR mice were randomly assigned into an HFD group and the HFD was supplemented with 0.1% (w/w) Bacillus subtilis B10 (HFD+B10 group). Thereafter, 30-d treatments were run, and then hepatic lipid level and antioxidant status were measured. The expression of genes related to lipid metabolism and oxidative stress in the liver was determined by reverse-transcription quantitative polymerase chain reaction (RT-qPCR). We found that HFD-induced obese mice treated with B10 showed a decrease in weight gain, serum glucose activity as well as hepatic triglyceride (TG), glutamic oxaloacetic transaminase (GOT), and glutamic pyruvic transaminase (GPT) activities. In addition, the gene expressions of antioxidant genes, glutathione reductase (GR), xanthine oxidase (XO), heat-shock protein 90 (Hsp90), and lipid synthesis gene 3β-hydroxysteroid-∆24 reductase (DHCR24) in the HFD+B10 group were down-regulated, suggesting alleviation of oxidative stress, while the lipolysis gene 3-hydroxy-3-methylglutaryl-CoA synthase 2 (HMGCS2), energy metabolism gene peroxisome proliferator-activated receptor α (PPARα) and the gene encoding tumor-suppressor protein p53 were up-regulated. The regulatory and positive effect of dietary supplementation of probiotic B10 suggests that it has a beneficial effect on the homeostasis of the lipid metabolism and on alleviating oxidative stress in HFD-induced obese mice.

Keywords: Bacillus subtilis; High-fat diet; Lipid metabolism; Oxidative stress.

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Conflict of interest statement

Compliance with ethics guidelines: Kai LEI, Ya-li LI, Yang WANG, Jing WEN, Hong-zhao WU, Dong-you YU, and Wei-fen LI declare that they have no conflict of interest.

All institutional and national guidelines for the care and use of laboratory animals were followed.

Figures

Fig. 1

Fig. 1

Effect of dietary supplementation of Bacillus subtilis B10 on the expression of mRNA associated with lipid metabolism in mice liver of the HFD (high-fat diet) and HFD+B10 (high-fat diet with 0.1% Bacillus subtilis B10) groups All mRNA quantification data are normalized to the reference gene. Gene expression levels are expressed as values relative to that of the HFD group. Data are expressed as mean±SD (_n_=6). Different letters indicate a statistically significant difference between groups (P<0.05)

Fig. 2

Fig. 2

Effect of dietary supplementation of Bacillus subtilis B10 on the expression of mRNA associated with antioxidation in mice liver of the HFD (high-fat diet) and HFD+B10 (high-fat diet with 0.1% Bacillus subtilis B10) groups All mRNA quantification data are normalized to the reference gene. Gene expression levels are expressed as values relative to that of the HFD group. Data are expressed as mean±SD (_n_=6). Different letters indicate a statistically significant difference between groups (P<0.05)

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