Amelioration of Isoproterenol-Induced Oxidative Damage in Rat Myocardium by Withania somnifera Leaf Extract - PubMed (original) (raw)

Amelioration of Isoproterenol-Induced Oxidative Damage in Rat Myocardium by Withania somnifera Leaf Extract

Md Ibrahim Khalil et al. Biomed Res Int. 2015.

Abstract

We investigated the protective role of Withania somnifera leaf extract (WSLEt) on isoproterenol- (ISO-) induced myocardial infarction (MI) in rats. Subcutaneous injection of ISO (85 mg/kg body weight (b.w.)) administered to rats for two consecutive days caused a significant increase in cardiac troponin I (cTnI) levels and serum lipid profiles, as well as the activities of some marker enzymes. In addition to these diagnostic markers, there were increased levels of lipid peroxidation (LPO) and decreased activities of enzymatic antioxidants (superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione reductase (GRx), and glutathione-S-transferase (GST)) in the myocardium. However, oral pretreatment (100 mg/kg b.w.) with WSLEt for 4 weeks elicited a significant cardioprotective activity by lowering the levels of cTnI, lipid profiles, and marker enzymes. The levels of LPO products were also significantly decreased. Elevated activities of antioxidant enzymes were also observed in rats pretreated with WSLEt. As further confirmed histopathologically, our findings strongly suggest that the cardioprotective effect of WSLEt on myocardium experiencing ISO-induced oxidative damage may be due to an augmentation of the endogenous antioxidant system and an inhibition of LPO in the myocardial membrane. We conclude that WSLEt confers some protection against oxidative damage in ISO-induced MI in rats.

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Figures

Figure 1

Figure 1

Schematic representation of experimental design of the study.

Figure 2

Figure 2

WSLEt ameliorates the oxidative damage caused by ISO as demonstrated by the changes in cTnI levels. Bars represent mean values ± SD (n = 10); bars with different letters represent significantly different mean values at p < 0.05. ND: not detected.

Figure 3

Figure 3

WSLEt ameliorates the oxidative damage caused by ISO as demonstrated by the changes in the cardiac marker enzyme activities. (a) The effects of WSLEt on CK-MB and LDH levels and (b) the effects of WSLEt on AST and ALT levels. Bars represent mean values ± SD (n = 10); bars with different letters represent significantly different mean values at p < 0.05.

Figure 4

Figure 4

(a) Sham group: normal control heart showing normal cardiac muscle fibers. (b) WSLEt + Sham group: WSLEt-treated (100 mg/kg) heart showing normal muscle fibers without any pathological changes. (c) WSLEt + ISO group: WSLEt-treated (100 mg/kg) + ISO-treated (85 mg/kg) heart showing no edematous intramuscular space and fewer inflammatory cells (black arrows). (d) ISO group: ISO-treated (85 mg/kg) heart showing cardiac muscle fibers with muscle separation (green arrows), edematous intramuscular space (red arrows), and inflammatory cells (black arrows).

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References

    1. Alam N., Hossain M., Mottalib M. A., Sulaiman S. A., Gan S. H., Khalil M. I. Methanolic extracts of Withania somnifera leaves, fruits and roots possess antioxidant properties and antibacterial activities. BMC Complementary and Alternative Medicine. 2012;12(1, article 175) doi: 10.1186/1472-6882-12-175. - DOI - PMC - PubMed
    1. Sangwan R., Chaurasiya N., Misra L., et al. Phytochemical variability in commercial herbal products and preparations of Withania somnifera (Ashwagandha) Current Science. 2004;86(3):461–464.
    1. Dhuley J. N. RETRACTED: adaptogenic and cardioprotective action of ashwagandha in rats and frogs. Journal of Ethnopharmacology. 2000;70(1):57–63. - PubMed
    1. Kulkarni S. K., George B., Mathur R. Protective effect of Withania somnifera root extract on electrographic activity in a lithium-pilocarpine model of status epilepticus. Phytotherapy Research. 1998;12(6):451–453.
    1. Alam N., Hossain M., Khalil M. I., Moniruzzaman M., Sulaiman S. A., Gan S. H. High catechin concentrations detected in Withania somnifera (ashwagandha) by high performance liquid chromatography analysis. BMC Complementary and Alternative Medicine. 2011;11, article 65 doi: 10.1186/1472-6882-11-65. - DOI - PMC - PubMed

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