Isolation and molecular characterization of a cDNA encoding the 23-kDa subunit of human RNA polymerase II - PubMed (original) (raw)
. 1989 Aug 5;264(22):13114-21.
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- PMID: 2753903
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Isolation and molecular characterization of a cDNA encoding the 23-kDa subunit of human RNA polymerase II
U K Pati et al. J Biol Chem. 1989.
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- Isolation and molecular characterization of a cDNA encoding the 23-kDa subunit of human RNA polymerase II.
Pati UK, Weissman SM. Pati UK, et al. J Biol Chem. 1991 Jul 15;266(20):13468. J Biol Chem. 1991. PMID: 2071613 No abstract available.
Abstract
We have shown that antibodies against native calf thymus RNA polymerase II and antibodies against its 23-kDa subunit cross-reacted with the 23-kDa subunit of human RNA polymerase II. Immunoglobin G (IgG) against the 23-kDa subunit of calf thymus RNA polymerase II inhibited transcription in vitro from the adenovirus major late promoter. By immunoscreening of a human placenta lambda gt11 cDNA library with IgG against native CT RNA polymerase II and with IgG against its 23-kDa subunit, we isolated and characterized a full length 1.2-kilobase cDNA. We also generated oligonucleotide probes from a sequence of amino acid residues obtained by a modified peptide microsequencing procedure. The cDNAs isolated both from oligoscreening and immunoscreening were identical. The amino acid sequence deduced from the nucleotide sequence analysis indicates a polypeptide of 197 amino acid (23 kDa). The in vitro translation product of human cDNA HP-23 was precipitated by IgG against the 23-kDa subunit of CT RNA polymerase II. The amino acid sequence deduced from HP-23 showed no obvious homology with Escherichia coli RNA polymerase subunits or with any of its sigma factors.
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