Identification and characterization of interferon signaling-related microRNAs in occult hepatitis B virus infection - PubMed (original) (raw)

Yiying Wang et al. Clin Epigenetics. 2017.

Abstract

Background: Occult hepatitis B virus infection (OBI) is an important risk factor of liver cirrhosis and hepatocellular carcinoma. Type 1 interferon (IFN) signaling-related miRNAs were significantly associated with hepatitis B virus (HBV) infection. However, the characteristics of serum IFN signaling-related miRNAs in OBI remain unclear. Therefore, this study aimed to analyze the expression levels of serum IFN signaling-related miRNAs in OBI and to evaluate their potential values for OBI diagnosis.

Methods: Twenty serum samples for training test (10 healthy controls and 10 OBI patients) and 438 validation serum samples from healthy controls, asymptomatic HBsAg carriers (ASC), and chronic hepatitis B (CHB) and OBI patients were collected. Expression levels of 32 IFN signaling-related miRNAs were analyzed in training and validation sets of samples using RT-qPCR.

Results: Among 32 IFN signaling-related miRNAs, decreased miR-122 levels and increased miR-130a levels were detected in training OBI samples. Furthermore, the results from validation test showed that the mean serum miR-122 and miR-130a level was 2.28 ± 0.96 and 3.11 ± 0.93 in OBI subjects, respectively. Compared to the healthy controls, ASC and CHB patients, miR-122 levels were significantly downregulated, while miR-130a levels were significantly upregulated in OBI patients. ROC analysis indicated that miR-122 + miR-130a could differentiate OBI from healthy controls, ASC, and CHB (≥ 0.87 of AUC).

Conclusions: Our study suggested that decreased serum miR-122 level and increased miR-130a level were significantly associated with OBI. Moreover, a combination of miR-122 and miR-130a could be served as a potential marker for OBI diagnosis.

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Conflict of interest statement

This study was approved by the ethics committee of Huai’an Fourth Hospital, Huai’an, China (Ethics Certification Number: hasy/2016ky002), and written informed consent was obtained from each participant.

Not applicable.

Competing interests

The authors declare that they have no competing interests.

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Figures

Fig. 1

Fig. 1

The mean ratio of OBI vs. HC in training samples. Mean ratio value < 1.0: downregulated; mean ratio value > 1.0: upregulated; mean ratio value = 1.0: no changes

Fig. 2

Fig. 2

Relative expression levels of serum miR-122 and miR-130a in healthy controls, OBI, ASC, and CHB patients

Fig. 3

Fig. 3

Receiver operating characteristic (ROC) curve analyses using serum miR-122, miR-130a, and miR-122 + miR-130a for discriminating OBI patients from healthy controls and ASC and CHB patients

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