Evaluation of Antibody-Dependent Enhancement of SARS-CoV Infection in Rhesus Macaques Immunized with an Inactivated SARS-CoV Vaccine - PubMed (original) (raw)

Evaluation of Antibody-Dependent Enhancement of SARS-CoV Infection in Rhesus Macaques Immunized with an Inactivated SARS-CoV Vaccine

Fan Luo et al. Virol Sin. 2018 Apr.

No abstract available

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Conflict of interest statement

Conflict of interest

The authors declare that they have no conflict of interest to disclose.

Animal and Human Rights Statement

All of the study protocols and procedures were approved by the Institutional Animal Care and Use Committee of Wuhan University.

Figures

Fig. 1

Fig. 1

Low levels of neutralizing antibodies induced by the inactivated vaccine SARS-CoV Z-1 do not trigger ADE following the challenge. A Rhesus macaques were immunized intramuscularly with 2.5 μg beta-propiolactone-inactivated whole SARS-CoV Z-1 vaccine or PBS as control, and boosted on day 7. The blood was collected at 0, 4, 6, 7, 8, 9 weeks post-immunization. Titers were expressed as the reciprocal of the dilution at which 50% of the plaques reduced completely in the virus-wells. Data were presented as the mean ± S.E. for each group. B On day 10 or 14 post-infection, the surviving monkeys from the three groups were euthanized and necropsied; the lungs were collected and fixed using 4% formaldehyde, sectioned, and subjected to hematoxylin and eosin staining. Magnification, ×200. The arrows refer to the widening of alveolar septa and the infiltration of inflammatory cells. C The nasopharyngeal swabs were collected on day 2 post-challenge. The presence of SARS-CoV polymerase gene in the swabs was confirmed by RT-PCR. No. 1, 2, 3, 4, 8, 9, 10, and 11 lanes are from immunized group; No. 7, 12, 15, and 16 lanes are from virus control group; No. 5, 6, 13, 14 lanes are the swabs from negative control group; M, molecular size marker; PC, purified virus as positive control; NC, without cDNA template. D The virus titers of the sera from the monkeys whose samples showed positive results in virus isolation were detected on days 5 and 7 post-challenge by TCID50. The values of the titer were expressed directly by the calculated number.

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References

    1. de Alwis R, Williams KL, Schmid MA, Lai CY, Patel B, Smith SA, Crowe JE, Wang WK, Harris E, de Silva AM. Dengue viruses are enhanced by distinct populations of serotype cross-reactive antibodies in human immune sera. PLoS Pathog. 2014;10:e1004386. doi: 10.1371/journal.ppat.1004386. - DOI - PMC - PubMed
    1. Enjuanes L, Dediego ML, Alvarez E, Deming D, Sheahan T, Baric R. Vaccines to prevent severe acute respiratory syndrome coronavirus-induced disease. Virus Res. 2008;133:45–62. doi: 10.1016/j.virusres.2007.01.021. - DOI - PMC - PubMed
    1. Jaume M, Yip MS, Cheung CY, Leung HL, Li PH, Kien F, Dutry I, Callendret B, Escriou N, Altmeyer R, Nal B, Daëron M, Bruzzone R, Peiris JS. Anti-severe acute respiratory syndrome coronavirus spike antibodies trigger infection of human immune cells via a pH- and cysteine protease-independent FcgammaR pathway. J Virol. 2011;85:10582–10597. doi: 10.1128/JVI.00671-11. - DOI - PMC - PubMed
    1. Jolly PE, Weiss HL. Neutralization and enhancement of HIV-1 infection by Sera from HIV-1 infected individuals who progress to disease at different rates. Virology. 2000;273:52–59. doi: 10.1006/viro.2000.0401. - DOI - PubMed
    1. Kam YW, Kien F, Roberts A, Cheung YC, Lamirande EW, Vogel L, Chu SL, Tse J, Guarner J, Zaki SR, Subbarao K, Peiris M, Nal B, Altmeyer R. Antibodies against trimeric S glycoprotein protect hamsters against SARS-CoV challenge despite their capacity to mediate FcgammaRII-dependent entry into B cells in vitro. Vaccine. 2007;25:729–740. doi: 10.1016/j.vaccine.2006.08.011. - DOI - PMC - PubMed

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