Production of colony-stimulating factor by tumor cells and the factor-mediated induction of suppressor cells - PubMed (original) (raw)
. 1988 Jul 15;141(2):699-708.
Affiliations
- PMID: 2968407
Production of colony-stimulating factor by tumor cells and the factor-mediated induction of suppressor cells
Y Tsuchiya et al. J Immunol. 1988.
Abstract
Spleen mononuclear cells of C3H/HeN mice were cultivated with mitomycin C-treated tumor cells, X5563, MH134, MM48, MM46, and FM3A/R, all of which were of syngeneic origin, in a medium containing normal syngeneic mouse serum but not FCS. There was a proliferative response to X5563, MH134, and MM48, but not to the two other tumor cells, MM46 and FM3A/R. The responder spleen cells were found to be nonadherent cells with a phenotype of Thy-1-L3T4-Lyt2-Ig-Macl-, which were neither mature T and B cells nor mature macrophage/granulocytes. It was also found that the proliferation of these nonadherent no-marker cells was mediated by tumor cell-derived soluble factors but not by direct stimulation with tumor cells. The responsible factor was a molecule(s) with a Mr of 23 to 25 kDa, which had a CSF activity inducing granulocyte (G)-, macrophage (M)- and G + M-colonies in the bone marrow cells. Neutralization tests of this factor-induced proliferation of spleen cells revealed that a major part of the factor may be GM-CSF or a molecule closely related to it. Incubation of spleen mononuclear cells with these GM-CSF-like tumor cell factors resulted in induction of myeloblastic/promyelocytic cells with a phenotype of Mac-1+2+Ia+ Thy-1-L3T4-Lyt2-Ig- in the spleen cell cultures, which could suppress mitogenic responses of the spleen cells to T and B cell mitogens. GM-CSF-like activity could also be detected in the serum of mice bearing X5563, MH134, and MM48, but not in those bearing MM46 and FM3A/R. Subcutaneous inoculation of C3H/HeN mice with these X5563, MH134, and MM48 tumor cells generated massive metastasis in the lung and lymph nodes, whereas MM46 and FM3A/R produced no macroscopic tumor cell metastasis. These results strongly suggest the possibility that in some tumor cell-host systems, a GM-CSF-like factor(s) produced constitutively by the tumor cells may play an important role in the development of tumor metastasis, mediating through suppression of lymphoid tissues of the host.
Similar articles
- Expansion of immunoregulatory macrophages by granulocyte-macrophage colony-stimulating factor derived from a murine mammary tumor.
Fu YX, Watson G, Jimenez JJ, Wang Y, Lopez DM. Fu YX, et al. Cancer Res. 1990 Jan 15;50(2):227-34. Cancer Res. 1990. PMID: 2136804 - [A correlation between the abilities of tumor cells to produce GM-CSF and form metastasis].
Kumagai K, Hatakeyama K, Takeda K, Tsuchiya Y, Rikiishi H. Kumagai K, et al. Gan To Kagaku Ryoho. 1989 Oct;16(10):3367-73. Gan To Kagaku Ryoho. 1989. PMID: 2679394 Review. Japanese. - [Tumor metastasis and growth factors].
Tsuruo T, Yamori T. Tsuruo T, et al. Gan To Kagaku Ryoho. 1989 Oct;16(10):3374-8. Gan To Kagaku Ryoho. 1989. PMID: 2679395 Review. Japanese.
Cited by
- NOX2-Derived Reactive Oxygen Species in Cancer.
Grauers Wiktorin H, Aydin E, Hellstrand K, Martner A. Grauers Wiktorin H, et al. Oxid Med Cell Longev. 2020 Nov 27;2020:7095902. doi: 10.1155/2020/7095902. eCollection 2020. Oxid Med Cell Longev. 2020. PMID: 33312338 Free PMC article. Review. - Myeloid-derived suppressor cells-a new therapeutic target to overcome resistance to cancer immunotherapy.
Chesney JA, Mitchell RA, Yaddanapudi K. Chesney JA, et al. J Leukoc Biol. 2017 Sep;102(3):727-740. doi: 10.1189/jlb.5VMR1116-458RRR. Epub 2017 May 25. J Leukoc Biol. 2017. PMID: 28546500 Free PMC article. Review. - On the origin of myeloid-derived suppressor cells.
Millrud CR, Bergenfelz C, Leandersson K. Millrud CR, et al. Oncotarget. 2017 Jan 10;8(2):3649-3665. doi: 10.18632/oncotarget.12278. Oncotarget. 2017. PMID: 27690299 Free PMC article. Review. - Exploiting the Immunomodulatory Properties of Chemotherapeutic Drugs to Improve the Success of Cancer Immunotherapy.
Kersten K, Salvagno C, de Visser KE. Kersten K, et al. Front Immunol. 2015 Oct 7;6:516. doi: 10.3389/fimmu.2015.00516. eCollection 2015. Front Immunol. 2015. PMID: 26500653 Free PMC article. Review. - Transcriptional regulation of myeloid-derived suppressor cells.
Condamine T, Mastio J, Gabrilovich DI. Condamine T, et al. J Leukoc Biol. 2015 Dec;98(6):913-22. doi: 10.1189/jlb.4RI0515-204R. Epub 2015 Sep 3. J Leukoc Biol. 2015. PMID: 26337512 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Research Materials