Membrane complement receptor type three (CR3) has lectin-like properties analogous to bovine conglutinin as functions as a receptor for zymosan and rabbit erythrocytes as well as a receptor for iC3b - PubMed (original) (raw)
. 1985 May;134(5):3307-15.
- PMID: 2984286
Membrane complement receptor type three (CR3) has lectin-like properties analogous to bovine conglutinin as functions as a receptor for zymosan and rabbit erythrocytes as well as a receptor for iC3b
G D Ross et al. J Immunol. 1985 May.
Abstract
Human leukocyte complement receptor type three (CR3) was shown to be lectin-like and to resemble bovine serum conglutinin (K) in that it bound to both iC3b and unopsonized yeast (Saccharomyces cerevisiae), and was inhibited by EDTA or N-acetyl-D-glucosamine (NADG). CR3 and K also bound to zymosan (Z), a yeast cell wall extract that contains primarily polysaccharide and no detectable protein. However, structural differences and the absence of K on bovine phagocytes indicated that CR3 was not the human homologue of bovine K. Phagocytic and respiratory responses to unopsonized Z were CR3 dependent because they were inhibited by monoclonal antibodies specific for the alpha-chain of CR3 and did not occur with phagocytes from patients with a genetic deficiency of CR3. The binding of CR3 to Z did not require opsonization of the Z with neutrophil-secreted C3, as Z binding and responses were not inhibited by Fab anti-C3. In addition, CR3-dependent binding of yeast occurred with neutrophils from which protein secretion was blocked by fixation with paraformaldehyde. Rabbit erythrocytes (RaE) also bound weakly to neutrophil CR3 and triggered ingestion. Anti-CR3 not only blocked the binding and ingestion of RaE but also blocked selectively the ingestion of RaEC3b without affecting the strong binding mediated by CR1. Even though sheep E and sheep EC3b were not ingested by neutrophils, a weak binding of CR3 to sheep E was suggested by the finding of 20 to 40% inhibition of sheep EAIgG ingestion by anti-CR3. Such inhibition was only observed in buffers that allowed activity of the CR3 binding site and not in buffers containing either EDTA or NADG. An apparently contradictory finding was that the weak CR3-dependent binding of Z triggered neutrophil ingestion and a superoxide burst, whereas the avid CR3-dependent binding of sheep EC3bi did not induce significant ingestion or a respiratory burst. Blocking studies with monoclonal antibodies specific for different epitopes of the alpha-chain of CR3 suggested that this might result from the presence of two distinct binding sites in CR3: one site for fixed iC3b that did not trigger functions, and a second function-triggering site for Z that did not bind to fixed iC3b.
Similar articles
- Generation of three different fragments of bound C3 with purified factor I or serum. II. Location of binding sites in the C3 fragments for factors B and H, complement receptors, and bovine conglutinin.
Ross GD, Newman SL, Lambris JD, Devery-Pocius JE, Cain JA, Lachmann PJ. Ross GD, et al. J Exp Med. 1983 Aug 1;158(2):334-52. doi: 10.1084/jem.158.2.334. J Exp Med. 1983. PMID: 6224880 Free PMC article. - Phagocytosis by human monocyte-derived macrophages. Independent function of receptors for C3b (CR1) and iC3b (CR3).
Newman SL, Devery-Pocius JE, Ross GD, Henson PM. Newman SL, et al. Complement. 1984;1(4):213-27. Complement. 1984. PMID: 6242391 - Collectins and collectin receptors in innate immunity.
Holmskov UL. Holmskov UL. APMIS Suppl. 2000;100:1-59. APMIS Suppl. 2000. PMID: 11021254 Review. - Structural aspects of collectins and receptors for collectins.
Hansen S, Holmskov U. Hansen S, et al. Immunobiology. 1998 Aug;199(2):165-89. doi: 10.1016/S0171-2985(98)80025-9. Immunobiology. 1998. PMID: 9777404 Review.
Cited by
- Preparation of Albatrellus ovinus β-Glucan Microparticles with Dectin-1a Binding Properties.
Ellefsen CF, Struzek AM, Scherließ R, Hiorth M, Samuelsen ABC. Ellefsen CF, et al. ACS Appl Bio Mater. 2023 May 15;6(5):1863-1872. doi: 10.1021/acsabm.3c00071. Epub 2023 Apr 19. ACS Appl Bio Mater. 2023. PMID: 37075203 Free PMC article. - Phagocytosis via complement receptor 3 enables microbes to evade killing by neutrophils.
Smirnov A, Daily KP, Gray MC, Ragland SA, Werner LM, Brittany Johnson M, Eby JC, Hewlett EL, Taylor RP, Criss AK. Smirnov A, et al. J Leukoc Biol. 2023 Jul 1;114(1):1-20. doi: 10.1093/jleuko/qiad028. J Leukoc Biol. 2023. PMID: 36882066 Free PMC article. - Sialylation regulates neutrophil transepithelial migration, CD11b/CD18 activation, and intestinal mucosal inflammatory function.
Azcutia V, Kelm M, Fink D, Cummings RD, Nusrat A, Parkos CA, Brazil JC. Azcutia V, et al. JCI Insight. 2023 Mar 8;8(5):e167151. doi: 10.1172/jci.insight.167151. JCI Insight. 2023. PMID: 36719745 Free PMC article. - Aspergillus fumigatus Induces the Release of IL-8 and MCP-1 by Activating Nuclear Transcription Through Dectin-1 and CR3 Receptors in Alveolar Epithelial Cells.
Liu Y, Li Z, Wang S, Zhang C, Han L, Sun Q, Han X. Liu Y, et al. Curr Microbiol. 2021 Sep;78(9):3474-3482. doi: 10.1007/s00284-021-02534-5. Epub 2021 Jul 16. Curr Microbiol. 2021. PMID: 34272600 - The Promiscuous Profile of Complement Receptor 3 in Ligand Binding, Immune Modulation, and Pathophysiology.
Lamers C, Plüss CJ, Ricklin D. Lamers C, et al. Front Immunol. 2021 Apr 29;12:662164. doi: 10.3389/fimmu.2021.662164. eCollection 2021. Front Immunol. 2021. PMID: 33995387 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Other Literature Sources
Miscellaneous