Comparison of the conformation of an oligonucleotide containing a central G-T base pair with the non-mismatch sequence by proton NMR - PubMed (original) (raw)
Comparative Study
. 1987 Apr 24;15(8):3397-409.
doi: 10.1093/nar/15.8.3397.
- PMID: 3033602
- PMCID: PMC340737
- DOI: 10.1093/nar/15.8.3397
Free PMC article
Comparative Study
Comparison of the conformation of an oligonucleotide containing a central G-T base pair with the non-mismatch sequence by proton NMR
E Quignard et al. Nucleic Acids Res. 1987.
Free PMC article
Abstract
We have recorded NOESY spectra of two non-selfcomplementary undecanucleotide duplexes. From the observed NOEs we do not detect any significant distortion of the helix when a G-C pair is replaced by a G-T pair and the normal interresidue connectivities can be followed through the mismatch site. We conclude that the 2D spectra of the non-exchangeable protons do not allow differentiation between a wobble or rare tautomer form for the mismatch. NOE measurements in H2O, however, clearly show that the mismatch adopts a wobble structure and give information on the hydration in the minor groove for the G-T base pair which is embedded between two A-T base pairs in the sequence.
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