Isolation and oncogenic potential of a novel human src-like gene - PubMed (original) (raw)
Isolation and oncogenic potential of a novel human src-like gene
T Kawakami et al. Mol Cell Biol. 1986 Dec.
Abstract
We have isolated cDNA molecules representing the complete coding sequence of a new human gene which is a member of the src family of oncogenes. Nucleotide sequence analysis revealed that this gene, termed slk, encoded a 537-residue protein which was 86% identical to the chicken proto-oncogene product, p60c-src, over a stretch of 191 amino acids at its carboxy terminus. In contrast, only 6% amino acid homology was observed within the amino-terminal 82 amino acid residues of these two proteins. It was possible to activate slk as a transforming gene by substituting approximately two-thirds of the slk coding sequence for an analogous region of the v-fgr onc gene present in Gardner-Rasheed feline sarcoma virus. The resulting hybrid protein molecule expressed in transformed cells demonstrated protein kinase activity with specificity for tyrosine residues.
References
- Proc Natl Acad Sci U S A. 1980 Mar;77(3):1311-5 - PubMed
- Important Adv Oncol. 1985;:3-15 - PubMed
- Virology. 1973 Apr;52(2):456-67 - PubMed
- Cell. 1977 May;11(1):223-32 - PubMed
- Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 - PubMed
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical
Molecular Biology Databases
Miscellaneous