Effect of tissue fixation on anti-bromodeoxyuridine immunohistochemistry - PubMed (original) (raw)

. 1987 Nov;35(11):1343-5.

doi: 10.1177/35.11.3116075.

Affiliations

Effect of tissue fixation on anti-bromodeoxyuridine immunohistochemistry

B Schutte et al. J Histochem Cytochem. 1987 Nov.

Abstract

We investigated the influence of various fixatives on monoclonal anti-BrdUrd antibody binding of BrdUrd-substituted DNA in tissue sections of routinely processed mouse small intestine after in vivo administration of BrdUrd. For denaturing fixatives such as ethanol or Carnoy's fluid, a standard denaturation protocol showed specific crypt cell labeling. With cross-linking agents such as formalin and glutaraldehyde, a remarkable increase in staining intensity was obtained after tissue digestion with pepsin before acid denaturation. The optimal pepsin concentration was determined for maximal immunoreactivity combined with acceptable morphology.

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