Peripheral immunophenotypes in children with multisystem inflammatory syndrome associated with SARS-CoV-2 infection - PubMed (original) (raw)

. 2020 Nov;26(11):1701-1707.

doi: 10.1038/s41591-020-1054-6. Epub 2020 Aug 18.

Matthew Fish # 3 4 5, Aislinn Jennings # 3 4, Katie J Doores 4, Paul Wellman 2, Jeffrey Seow 4, Sam Acors 4, Carl Graham 4, Emma Timms 5, Julia Kenny 1 2, Stuart Neil 4, Michael H Malim 4, Shane M Tibby 6, Manu Shankar-Hari 7 8 9

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Peripheral immunophenotypes in children with multisystem inflammatory syndrome associated with SARS-CoV-2 infection

Michael J Carter et al. Nat Med. 2020 Nov.

Abstract

Recent reports highlight a new clinical syndrome in children related to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)1-multisystem inflammatory syndrome in children (MIS-C)-which comprises multiorgan dysfunction and systemic inflammation2-13. We performed peripheral leukocyte phenotyping in 25 children with MIS-C, in the acute (n = 23; worst illness within 72 h of admission), resolution (n = 14; clinical improvement) and convalescent (n = 10; first outpatient visit) phases of the illness and used samples from seven age-matched healthy controls for comparisons. Among the MIS-C cohort, 17 (68%) children were SARS-CoV-2 seropositive, suggesting previous SARS-CoV-2 infections14,15, and these children had more severe disease. In the acute phase of MIS-C, we observed high levels of interleukin-1β (IL-1β), IL-6, IL-8, IL-10, IL-17, interferon-γ and differential T and B cell subset lymphopenia. High CD64 expression on neutrophils and monocytes, and high HLA-DR expression on γδ and CD4+CCR7+ T cells in the acute phase, suggested that these immune cell populations were activated. Antigen-presenting cells had low HLA-DR and CD86 expression, potentially indicative of impaired antigen presentation. These features normalized over the resolution and convalescence phases. Overall, MIS-C presents as an immunopathogenic illness1 and appears distinct from Kawasaki disease.

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References

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