Purified maturation-promoting factor contains the product of a Xenopus homolog of the fission yeast cell cycle control gene cdc2+ - PubMed (original) (raw)
Purified maturation-promoting factor contains the product of a Xenopus homolog of the fission yeast cell cycle control gene cdc2+
J Gautier et al. Cell. 1988.
Abstract
In the fission yeast S. pombe, the Mr = 34 kd product of the cdc2+ gene (p34cdc2) is a protein kinase that controls entry into mitosis. In Xenopus oocytes and other cells, maturation-promoting factor (MPF) appears in late G2 phase and is able to cause entry into mitosis. Purified MPF consists of two major proteins of Mr approximately equal to 32 kd and 45 kd and expresses protein kinase activity. We report here that antibodies to S. pombe p34cdc2 are able to immunoblot and immunoprecipitate the approximately equal to 32 kd component of MPF from Xenopus eggs. The Mr approximately equal to 32 kd and 45 kd proteins exist as a complex that expresses protein kinase activity. These findings indicate that a Xenopus p34cdc2 homolog is present in purified MPF and suggest that p34cdc2 is a component of the control mechanism initiating mitosis generally in eukaryotic cells.
Similar articles
- The Xenopus cdc2 protein is a component of MPF, a cytoplasmic regulator of mitosis.
Dunphy WG, Brizuela L, Beach D, Newport J. Dunphy WG, et al. Cell. 1988 Jul 29;54(3):423-31. doi: 10.1016/0092-8674(88)90205-x. Cell. 1988. PMID: 3293802 - Dephosphorylation and activation of Xenopus p34cdc2 protein kinase during the cell cycle.
Gautier J, Matsukawa T, Nurse P, Maller J. Gautier J, et al. Nature. 1989 Jun 22;339(6226):626-9. doi: 10.1038/339626a0. Nature. 1989. PMID: 2543932 - Controls of cell proliferation in yeast and animals.
Norbury C, Nurse P. Norbury C, et al. Ciba Found Symp. 1990;150:168-77; discussion 177-83. doi: 10.1002/9780470513927.ch11. Ciba Found Symp. 1990. PMID: 2197066 Review. - [From ovocyte to biochemistry of the cell cycle].
Ozon R. Ozon R. Verh K Acad Geneeskd Belg. 1991;53(4):365-85. Verh K Acad Geneeskd Belg. 1991. PMID: 1659057 Review. French.
Cited by
- Japanese encephalitis virus NS1 and NS1' proteins induce vimentin rearrangement via the CDK1-PLK1 axis to promote viral replication.
Xie S, Yang X, Yang X, Cao Z, Wei N, Lin X, Shi M, Cao R. Xie S, et al. J Virol. 2024 May 14;98(5):e0019524. doi: 10.1128/jvi.00195-24. Epub 2024 Apr 24. J Virol. 2024. PMID: 38656209 - Combined evaluation of both WEE1 and phosphorylated cyclin dependent kinase 1 expressions in oral squamous cell carcinomas predicts cancer recurrence and progression.
Wu YH, Yu-Fong Chang J, Chiang CP, Wang YP. Wu YH, et al. J Dent Sci. 2022 Oct;17(4):1780-1787. doi: 10.1016/j.jds.2022.08.009. Epub 2022 Aug 26. J Dent Sci. 2022. PMID: 36299328 Free PMC article. - Chemical-Induced Premature Chromosome Condensation Protocol.
Gotoh E. Gotoh E. Methods Mol Biol. 2023;2519:41-51. doi: 10.1007/978-1-0716-2433-3_5. Methods Mol Biol. 2023. PMID: 36066708 - Explaining Redundancy in CDK-Mediated Control of the Cell Cycle: Unifying the Continuum and Quantitative Models.
Fisher D, Krasinska L. Fisher D, et al. Cells. 2022 Jun 24;11(13):2019. doi: 10.3390/cells11132019. Cells. 2022. PMID: 35805103 Free PMC article. Review. - A Journey through Time on the Discovery of Cell Cycle Regulation.
Uzbekov R, Prigent C. Uzbekov R, et al. Cells. 2022 Feb 17;11(4):704. doi: 10.3390/cells11040704. Cells. 2022. PMID: 35203358 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Miscellaneous