Purification to homogeneity and NH2-terminal amino acid sequence of a novel interleukin 1 species derived from a human B cell line - PubMed (original) (raw)
. 1986 May 1;136(9):3304-10.
- PMID: 3485686
Purification to homogeneity and NH2-terminal amino acid sequence of a novel interleukin 1 species derived from a human B cell line
L Rimsky et al. J Immunol. 1986.
Abstract
A subclone, referred to as 3B6, derived from a DR-negative EBV-transformed B cell line, has been found to spontaneously produce IL 1. 3B6-IL 1 displays a pI of 5 on FPLC chromatofocusing. It has been purified to homogeneity by a sequence of ion-exchange chromatography and affinity chromatography on procion red agarose. The homogeneous material migrated with an apparent m.w. of 13,500 on SDS-PAGE. The overall recovery of IL 1 activity was estimated at 57%. The final material had a specific activity of 7.8 X 10(6) half-maximal units/mg and represented a 50,000-fold purification. A partial NH2-terminal amino acid sequence has been obtained that is different from those reported from monocytic IL 1. However, this molecule can formally be identified as IL 1 on its spectrum of biologic activities. In addition to inducing the proliferation of murine thymocytes in the co-stimulator assay. 3B6-IL 1 is active on both human T and B cells, respectively, in inducing IL 2 synthesis by cells from a subcloned HSB 2 line and promoting the proliferation of anti-IgM-stimulated human peripheral blood B lymphocytes. Furthermore, 3B6-IL 1 acts as a growth factor for normal human fibroblasts and for the 3B6 line itself. However, 3B6-IL 1 is not pyrogenic in rabbits. Thus, the 3B6 cell line was shown to produce a new molecular species of IL 1, with respect to its NH2-terminal sequence, which shared all of the studied biologic activities of monocytic IL 1 except for pyrogenicity.
Similar articles
- Purification and characterization of a unique human interleukin 1 from the tumor cell line U937.
Knudsen PJ, Dinarello CA, Strom TB. Knudsen PJ, et al. J Immunol. 1986 May 1;136(9):3311-6. J Immunol. 1986. PMID: 3485687 - B-cell line-derived interleukin 1 is cytotoxic for melanoma cells and promotes the proliferation of an astrocytoma cell line.
Bertoglio JH, Rimsky L, Kleinerman ES, Lachman LB. Bertoglio JH, et al. Lymphokine Res. 1987 Spring;6(2):83-91. Lymphokine Res. 1987. PMID: 3495709 - IL-2 receptor(p55)/Tac-inducing factor. Purification and characterization of adult T cell leukemia-derived factor.
Tagaya Y, Okada M, Sugie K, Kasahara T, Kondo N, Hamuro J, Matsushima K, Dinarello CA, Yodoi J. Tagaya Y, et al. J Immunol. 1988 Apr 15;140(8):2614-20. J Immunol. 1988. PMID: 2895791 - [Interleukin 1: a new hormone].
Ramadori G, Rieder H, Meuer S, Meyer zum Büschenfelde KH. Ramadori G, et al. Dtsch Med Wochenschr. 1986 Jun 27;111(26):1032-8. doi: 10.1055/s-2008-1068579. Dtsch Med Wochenschr. 1986. PMID: 2424704 Review. German. No abstract available.
Cited by
- Human plasma accelerates immortalization of B lymphocytes by Epstein-Barr virus.
Manor E. Manor E. Cell Prolif. 2008 Apr;41(2):292-8. doi: 10.1111/j.1365-2184.2008.00513.x. Cell Prolif. 2008. PMID: 18336473 Free PMC article. - Post-transplant lymphoproliferative disease (PTLD): lymphokine production and PTLD.
Tosato G, Teruya-Feldstein J, Setsuda J, Pike SE, Jones KD, Jaffe ES. Tosato G, et al. Springer Semin Immunopathol. 1998;20(3-4):405-23. doi: 10.1007/BF00838052. Springer Semin Immunopathol. 1998. PMID: 9870254 Review. No abstract available. - Lymphotoxin is an autocrine growth factor for Epstein-Barr virus-infected B cell lines.
Estrov Z, Kurzrock R, Pocsik E, Pathak S, Kantarjian HM, Zipf TF, Harris D, Talpaz M, Aggarwal BB. Estrov Z, et al. J Exp Med. 1993 Mar 1;177(3):763-74. doi: 10.1084/jem.177.3.763. J Exp Med. 1993. PMID: 8382256 Free PMC article. - Oncostatin M: a growth regulator produced by differentiated histiocytic lymphoma cells.
Zarling JM, Shoyab M, Marquardt H, Hanson MB, Lioubin MN, Todaro GJ. Zarling JM, et al. Proc Natl Acad Sci U S A. 1986 Dec;83(24):9739-43. doi: 10.1073/pnas.83.24.9739. Proc Natl Acad Sci U S A. 1986. PMID: 3540948 Free PMC article. - The marmoset B-lymphoblastoid cell line (B95-8) produces and responds to B-cell growth and differentiation factors: role of shed CD23 (sCD23).
Callard RE, Lau YL, Shields JG, Smith SH, Cairns J, Flores-Romo L, Gordon J. Callard RE, et al. Immunology. 1988 Nov;65(3):379-84. Immunology. 1988. PMID: 3145248 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Other Literature Sources
Research Materials
Miscellaneous