Circadian rhythmicity restored by neural transplant. Immunocytochemical characterization of the graft and its integration with the host brain - PubMed (original) (raw)

Circadian rhythmicity restored by neural transplant. Immunocytochemical characterization of the graft and its integration with the host brain

M N Lehman et al. J Neurosci. 1987 Jun.

Abstract

It is well established that overt circadian rhythms are permanently disrupted following lesions of the hamster hypothalamic suprachiasmatic nucleus (SCN). In the present study, we show that implantations of brain grafts containing the fetal SCN reestablish circadian rhythms of locomotor activity in adult hamsters previously made arrhythmic by SCN lesions. The restoration of free-running rhythms in conditions of constant darkness is correlated with the presence in the graft of neuropeptides normally present in the SCN of unlesioned hamsters, including vasoactive intestinal polypeptide (VIP), neuropeptide Y (NPY), and vasopressin (VP). In several recipients, grafts were found to receive retinal input, and appeared to send efferents into the host brain. Not all functions of the SCN were reinstated by the graft: animals with restored locomotor rhythms did not show gonadal regression in the absence of light, and failed to synchronize (entrain) to light intensities to which SCN-intact animals responded.

PubMed Disclaimer

Cited by

Age-related disruptions of circadian rhythm and memory in the senescence-accelerated mouse (SAMP8).
Pang KC, Miller JP, Fortress A, McAuley JD. Pang KC, et al. Age (Dordr). 2006 Sep;28(3):283-96. doi: 10.1007/s11357-006-9013-9. Epub 2006 Nov 23. Age (Dordr). 2006. PMID: 22253495 Free PMC article.
Understanding Quantitative Circadian Regulations Are Crucial Towards Advancing Chronotherapy.
Chowdhury D, Wang C, Lu AP, Zhu HL. Chowdhury D, et al. Cells. 2019 Aug 13;8(8):883. doi: 10.3390/cells8080883. Cells. 2019. PMID: 31412622 Free PMC article. Review.
Circadian lessons from peripheral clocks: is the time of the mammalian pacemaker up?
Brandstaetter R. Brandstaetter R. Proc Natl Acad Sci U S A. 2004 Apr 20;101(16):5699-700. doi: 10.1073/pnas.0401378101. Epub 2004 Apr 12. Proc Natl Acad Sci U S A. 2004. PMID: 15079063 Free PMC article. No abstract available.
Mammalian circadian clock and metabolism - the epigenetic link.
Bellet MM, Sassone-Corsi P. Bellet MM, et al. J Cell Sci. 2010 Nov 15;123(Pt 22):3837-48. doi: 10.1242/jcs.051649. J Cell Sci. 2010. PMID: 21048160 Free PMC article. Review.
Anatomical Methods to Study the Suprachiasmatic Nucleus.
Bittman EL. Bittman EL. Methods Mol Biol. 2022;2482:191-210. doi: 10.1007/978-1-0716-2249-0_13. Methods Mol Biol. 2022. PMID: 35610428 Free PMC article.

Circadian rhythmicity restored by neural transplant. Immunocytochemical characterization of the graft and its integration with the host brain - PubMed (original) (raw)