C3 leukotactic factors produced by a tissue protease - PubMed (original) (raw)

C3 leukotactic factors produced by a tissue protease

J H Hill et al. J Exp Med. 1969.

Abstract

When various rat tissues are incubated in homologous serum, a factor chemotactic in vitro for neutrophils is generated. The amount of chemotactic activity is a function of duration of incubation and the quantity of heart tissue or serum employed. Addition of trypsin inhibitor or antibody to the third component of complement (C3) precludes generation of chemotactic activity. In addition, antibody to C3 ablates chemotactic activity even after its formation. Purified human C3 (beta(1C)-globulin) effectively substitutes for serum in the generation of chemotactic activity by heart tissue. The active product, as determined by gel filtration or by ultracentrifugal analysis in a sucrose density gradient, appears to be a cleavage product of C3 with a molecular weight of approximately 14,000. In addition, a larger C3 fragmentation product varying in molecular weight, depending upon experimental conditions, is also found. The protease in rat heart tissue capable of cleaving C3 into chemotactic fragments is a serine esterase with trypsin-like properties and can be inhibited by organophophorous compounds or trypsin inhibitors. The use of amino acid esters in the manner of competitive substrate inhibition confirms the trypsin-like nature of the protease. The presence of a protease in heart, and presumably in other normal tissues, capable of fragmenting C3 into factors with chemotactic activities may explain the development of the acute inflammatory response when tissues are non-specifically injured. If true, this would reinforce the role of the complement system in the mediation of nonimmunologically induced inflammation.

PubMed Disclaimer

Similar articles

• The phlogistic role of C3 leukotactic fragments in myocardial infarcts of rats.
  Hill JH, Ward PA. Hill JH, et al. J Exp Med. 1971 Apr 1;133(4):885-900. doi: 10.1084/jem.133.4.885. J Exp Med. 1971. PMID: 4993831 Free PMC article.
• The deactivation of rabbit neutrophils by chemotactic factor and the nature of the activatable esterase.
  Ward PA, Becker EL. Ward PA, et al. J Exp Med. 1968 Apr 1;127(4):693-709. doi: 10.1084/jem.127.4.693. J Exp Med. 1968. PMID: 5642465 Free PMC article.
• Relationship between the C5 peptides chemotactic for leukocytes and tumor cells.
  Romualdez AG, Ward PA, Torikata T. Romualdez AG, et al. J Immunol. 1976 Nov;117(5 Pt.2):1762-6. J Immunol. 1976. PMID: 62790
• Structural features and biologic properties of fragments obtained by limited proteolysis of C3.
  Conroy MC, Ozols J, Lepow IH. Conroy MC, et al. J Immunol. 1976 Jun;116(6):1682-7. J Immunol. 1976. PMID: 774990
• Enzyme activation and the mechanism of neutrophil chemotaxis.
  Becker EL. Becker EL. Antibiot Chemother (1971). 1974;19:409-20. Antibiot Chemother (1971). 1974. PMID: 4142851 Review. No abstract available.

Cited by

• Complement activation and prognosis in patients with type 2 diabetes and myocardial infarction: a report from the DIGAMI 2 trial.
  Mellbin LG, Bjerre M, Thiel S, Hansen TK. Mellbin LG, et al. Diabetes Care. 2012 Apr;35(4):911-7. doi: 10.2337/dc11-1642. Epub 2012 Feb 22. Diabetes Care. 2012. PMID: 22357179 Free PMC article.
• Chemotaxis and its significance for leucocyte accumulation.
  Keller HU. Keller HU. Agents Actions. 1972 Jun;2(4):161-9. doi: 10.1007/BF01965854. Agents Actions. 1972. PMID: 4559311 Review. No abstract available.
• The phlogistic role of C3 leukotactic fragments in myocardial infarcts of rats.
  Hill JH, Ward PA. Hill JH, et al. J Exp Med. 1971 Apr 1;133(4):885-900. doi: 10.1084/jem.133.4.885. J Exp Med. 1971. PMID: 4993831 Free PMC article.
• Experimental cytomegalovirus ophthalmitis.
  Schwartz JN, Daniels CA, Shivers JC, Klintworth GK. Schwartz JN, et al. Am J Pathol. 1974 Dec;77(3):477-92. Am J Pathol. 1974. PMID: 4372891 Free PMC article.
• The alternate pathway of complement activation. The role of C3 and its inactivator (KAF).
  Nicol PA, Lachmann PJ. Nicol PA, et al. Immunology. 1973 Feb;24(2):259-75. Immunology. 1973. PMID: 4632688 Free PMC article.

References

1. 1. Biochem J. 1965 Sep;96(3):595-606 - PubMed
2. 1. Immunology. 1966 Aug;11(2):141-53 - PubMed
3. 1. J Immunol. 1966 Nov;97(5):680-5 - PubMed
4. 1. Science. 1967 Mar 3;155(3766):1122-3 - PubMed
5. 1. J Exp Med. 1967 Jun 1;125(6):1021-30 - PubMed

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Europe PubMed Central
  • Ovid Technologies, Inc.
  • PubMed Central
  • Silverchair Information Systems

• Miscellaneous

  • NCI CPTAC Assay Portal