Stimulation of steroid secretion in adrenal tumor cells by choleragen - PubMed (original) (raw)
Stimulation of steroid secretion in adrenal tumor cells by choleragen
J Wolff et al. Proc Natl Acad Sci U S A. 1973 Oct.
Abstract
Choleragen, the pure protein from cholera toxin, stimulates steroid secretion by Y-1 adrenal tumor cells in culture. The secreted steroids are the same as seen after addition of adrenocorticotropic hormone. Half-maximal stimulation occurs at 15 pM; stimulation is essentially irreversible by washing and partially reversible (for about 1 hr) by antibody, and there is a latent period of about 60 min before stimulation is seen. Stimulation of adenylate cyclase occurs at about 30-fold higher choleragen concentrations. Gangliosides inhibit choleragen stimulation when added before but not after the toxin. Lipopolysaccharides from Escherichia coli, Salmonella typhosa, and Serratia marcescens also stimulate steroid secretion, but are less potent than choleragen.
Similar articles
- Choleragen stimulates steroidogenesis and adenylate cyclase in cells lacking functional hormone receptors.
Wolff J, Cook GH. Wolff J, et al. Biochim Biophys Acta. 1975 Dec 1;413(2):283-90. doi: 10.1016/0005-2736(75)90113-3. Biochim Biophys Acta. 1975. PMID: 172154 - Mechanism of activation of adenylate cyclase by Vibrio cholerae enterotoxin. Relations to the mode of activation by hormones.
Bennett V, Mong L, Cuatrecasas P. Bennett V, et al. J Membr Biol. 1975 Nov 7;24(2):107-29. doi: 10.1007/BF01868618. J Membr Biol. 1975. PMID: 172636 - Endotoxic lipopolysaccharides stimulate steroidogenesis and adenylate cyclase in adrenal tumor cells.
Wolff J, Cook GH. Wolff J, et al. Biochim Biophys Acta. 1975 Dec 1;413(2):291-7. doi: 10.1016/0005-2736(75)90114-5. Biochim Biophys Acta. 1975. PMID: 172155 - The effect of cholera enterotoxin on steroidogenesis in cultured adrenal tumor cells.
Wishnow RM, Feist P. Wishnow RM, et al. J Infect Dis. 1974 Jun;129(6):690-5. doi: 10.1093/infdis/129.6.690. J Infect Dis. 1974. PMID: 4365946 No abstract available. - Activation of adenylate cyclase by choleragen.
Moss J, Vaughan M. Moss J, et al. Annu Rev Biochem. 1979;48:581-600. doi: 10.1146/annurev.bi.48.070179.003053. Annu Rev Biochem. 1979. PMID: 224804 Review. No abstract available.
Cited by
- 5'-Guanylylimidodiphosphate, a potent activator of adenylate cyclase systems in eukaryotic cells.
Londos C, Salomon Y, Lin MC, Harwood JP, Schramm M, Wolff J, Rodbell M. Londos C, et al. Proc Natl Acad Sci U S A. 1974 Aug;71(8):3087-90. doi: 10.1073/pnas.71.8.3087. Proc Natl Acad Sci U S A. 1974. PMID: 4607368 Free PMC article. - Mobility of cholera toxin receptors on rat lymphocyte membranes.
Craig SW, Cuatrecasas P. Craig SW, et al. Proc Natl Acad Sci U S A. 1975 Oct;72(10):3844-8. doi: 10.1073/pnas.72.10.3844. Proc Natl Acad Sci U S A. 1975. PMID: 1060063 Free PMC article. - Cholera toxin mimics melanocyte stimulating hormone in inducing differentiation in melanoma cells.
O'Keefe E, Cuatrecasas P. O'Keefe E, et al. Proc Natl Acad Sci U S A. 1974 Jun;71(6):2500-4. doi: 10.1073/pnas.71.6.2500. Proc Natl Acad Sci U S A. 1974. PMID: 4366771 Free PMC article. - Mechanism of action of Vibrio cholerae enterotoxin. Effects on adenylate cyclase of toad and rat erythrocyte plasma membranes.
Bennett V, Cuatrecasas P. Bennett V, et al. J Membr Biol. 1975 Jun 3;22(1):1-28. doi: 10.1007/BF01868161. J Membr Biol. 1975. PMID: 805247 - Adenosine 3',5'-cyclic monophosphate and adrenal tumor cell morphology.
Kwan CN, Wishnow RM. Kwan CN, et al. In Vitro. 1974 Nov-Dec;10(5-6):268-73. doi: 10.1007/BF02615307. In Vitro. 1974. PMID: 4375665 No abstract available.
References
- Nature. 1970 May 16;226(5246):658-9 - PubMed
- J Clin Invest. 1973 Mar;52(3):691-7 - PubMed
- Nature. 1973 Feb 9;241(5389):399 - PubMed
- J Clin Invest. 1973 Mar;52(3):698-708 - PubMed
- Gastroenterology. 1971 Jan;60(1):22-32 - PubMed
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials