An improved method for the isolation from Naja naja venom of cobra factor (CoF) free of phospholipase A - PubMed (original) (raw)
An improved method for the isolation from Naja naja venom of cobra factor (CoF) free of phospholipase A
M B Pepys et al. J Immunol Methods. 1979.
Abstract
An improved method is reported for the isolation from cobra (Naja naja) venom of cobra factor (CoF), the anticomplementary protein which is derived from cobra C3. Sequential chromatography on DEAE-Sepharose, Sephacryl-S200, and finally hydroxylapatite yielded 6.25 mg CoF per gram of crude venom. The purified CoF had 1 unit of functional anticomplementary activity per 1--2 micrograms of protein, and was homogeneous on gradient and non-reduced sodium dodecyl sulphate (SDS) polyacrylamide gel electrophoresis (PAGE). In SDS-PAGE after reduction with mercaptoethanol there were two major bands (M.W. 75,000 and 51,000 daltons), three minor bands (M.W. 29--31,500 daltons) and two trace bands (36,500 and 41,500 daltons). By analogy with mammalian C3 it is suggested that the CoF consists of two polypeptide chains linked by disulphide bridges, one of which undergoes cleavage of the peptide chain at several points either in vivo or in vitro.
Similar articles
- Purification and characterization of anticomplement factor (cobra venom factor) from the Naja naja atra venom.
Takahashi H, Hayashi K. Takahashi H, et al. Biochim Biophys Acta. 1982 Feb 4;701(1):102-10. doi: 10.1016/0167-4838(82)90317-x. Biochim Biophys Acta. 1982. PMID: 6173071 - A one-step isolation procedure for phospholipase A2-free cobra venom factor by fast protein liquid chromatography.
Beukelman CJ, Aerts PC, Van Dijk H, Willers JM. Beukelman CJ, et al. J Immunol Methods. 1987 Feb 26;97(1):119-22. doi: 10.1016/0022-1759(87)90113-x. J Immunol Methods. 1987. PMID: 3819435 - Cobra venom phospholipase A2: Naja naja naja.
Reynolds LJ, Dennis EA. Reynolds LJ, et al. Methods Enzymol. 1991;197:359-65. doi: 10.1016/0076-6879(91)97161-q. Methods Enzymol. 1991. PMID: 2051932 No abstract available. - Affinity chromatography of phospholipase A2 from Naja naja naja (Indian cobra) venom.
Hazlett TL, Dennis EA. Hazlett TL, et al. Toxicon. 1985;23(3):457-66. doi: 10.1016/0041-0101(85)90029-7. Toxicon. 1985. PMID: 3927524
Cited by
- Classical and alternative pathway complement activation are not required for reactive systemic AA amyloid deposition in mice.
Hutchinson WL, Herbert J, Botto M, Pepys MB. Hutchinson WL, et al. Immunology. 2004 Jun;112(2):250-4. doi: 10.1111/j.1365-2567.2004.01881.x. Immunology. 2004. PMID: 15147568 Free PMC article. - C-reactive protein and complement are important mediators of tissue damage in acute myocardial infarction.
Griselli M, Herbert J, Hutchinson WL, Taylor KM, Sohail M, Krausz T, Pepys MB. Griselli M, et al. J Exp Med. 1999 Dec 20;190(12):1733-40. doi: 10.1084/jem.190.12.1733. J Exp Med. 1999. PMID: 10601349 Free PMC article. - Trypanosoma cruzi infection in rats induced early lesion of the heart noradrenergic nerve terminals by a complement-independent mechanism.
Machado CR, de Oliveira DA, Magalhaes MJ, Carvalho EM, Ramalho-Pinto FJ. Machado CR, et al. J Neural Transm Gen Sect. 1994;97(2):149-59. doi: 10.1007/BF01277950. J Neural Transm Gen Sect. 1994. PMID: 7873124 - Isolation and characterization of C-reactive protein and serum amyloid P component in the rat.
de Beer FC, Baltz ML, Munn EA, Feinstein A, Taylor J, Bruton C, Clamp JR, Pepys MB. de Beer FC, et al. Immunology. 1982 Jan;45(1):55-70. Immunology. 1982. PMID: 7056568 Free PMC article. - Role of the acute phase response in the Shwartzman phenomenon.
Pepys MB, Rogers SL, Evans DJ. Pepys MB, et al. Clin Exp Immunol. 1982 Feb;47(2):289-95. Clin Exp Immunol. 1982. PMID: 7042139 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Miscellaneous