Rearrangement and amplification of c-abl sequences in the human chronic myelogenous leukemia cell line K-562 - PubMed (original) (raw)

Comparative Study

Rearrangement and amplification of c-abl sequences in the human chronic myelogenous leukemia cell line K-562

S J Collins et al. Proc Natl Acad Sci U S A. 1983 Aug.

Abstract

Structural rearrangements of specific cellular sequences (c-onc genes) homologous to acute transforming retrovirus oncogenes (v-onc genes) have been recently described in various malignancies. Here we show that human cellular sequences (c-abl) homologous to the transforming sequences of the mouse Abelson leukemia virus (v-abl) are amplified some 4- to 8-fold in K-562, a Philadelphia chromosome-positive cell line derived from a patient with chronic myelogenous leukemia in blast crisis. Restriction analysis of K-562 and other human DNA samples reveals a significant rearrangements of the c-abl sequences in this cell line. In addition, investigation of v-abl-related cytoplasmic RNA reveals relatively high levels of these sequences in K-562 compared to other normal and leukemia cells. We have also observed that lambda light chain constant region immunoglobulin genes are amplified in K-562, whereas kappa light chain sequences exhibit no amplification. These results are discussed within the context of the possibility that these Philadelphia chromosome-positive cells exhibit a reciprocal translocation involving chromosome 9 (containing c-abl) and chromosome 22 (containing the lambda light chain genes).

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References

1. 1. Lancet. 1971 Oct 30;2(7731):971-2 - PubMed
2. 1. Cell. 1982 Dec;31(2 Pt 1):443-52 - PubMed
3. 1. Blood. 1975 Mar;45(3):321-34 - PubMed
4. 1. J Mol Biol. 1975 Nov 5;98(3):503-17 - PubMed
5. 1. Int J Cancer. 1976 Oct 15;18(4):421-31 - PubMed

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