Laminin and fibronectin promote the haptotactic migration of B16 mouse melanoma cells in vitro - PubMed (original) (raw)

Laminin and fibronectin promote the haptotactic migration of B16 mouse melanoma cells in vitro

J B McCarthy et al. J Cell Biol. 1984 Apr.

Abstract

The migration of tumor cells through basement membranes and extracellular matrices is an integral component of tumor invasion and metastasis. Laminin and fibronectin are two basement membrane- and extracellular matrix-associated noncollagenous glycoproteins that have been shown to promote both cell adhesion and motility. Purified preparations of laminin and fibronectin stimulated the directed migration of B16 murine metastatic melanoma cells in vitro as assessed in modified Boyden chambers. The stimulation of migration occurred over a concentration range of 1-100 micrograms/ml of laminin or fibronectin, with a peak response occurring between 12.5 and 25 micrograms/ml. The maximal response of these cells was 80-120-fold higher than control migration. Affinity-purified antibody preparations specifically abrogated the migration of these cells in response to the respective proteins. Tumor cells in suspension were preincubated in physiologic levels of plasma fibronectin prior to assay to partially mimic what occurs when a metastasizing cell is in the blood stream. This preincubation with plasma fibronectin had no effect on the subsequent migration of cells in response to either laminin or fibronectin. Furthermore, experiments using filters precoated with fibronectin or laminin indicated that these cells could migrate by haptotaxis to these two proteins. We conclude that tumor cell migration in response to such noncollagenous adhesive glycoproteins could be an important aspect in the invasion and metastasis of certain malignant cell types.

PubMed Disclaimer

Cited by

Thrombospondin-induced tumor cell migration: haptotaxis and chemotaxis are mediated by different molecular domains.
Taraboletti G, Roberts DD, Liotta LA. Taraboletti G, et al. J Cell Biol. 1987 Nov;105(5):2409-15. doi: 10.1083/jcb.105.5.2409. J Cell Biol. 1987. PMID: 3680388 Free PMC article.
The role of cell adhesion proteins--laminin and fibronectin--in the movement of malignant and metastatic cells.
McCarthy JB, Basara ML, Palm SL, Sas DF, Furcht LT. McCarthy JB, et al. Cancer Metastasis Rev. 1985;4(2):125-52. doi: 10.1007/BF00050692. Cancer Metastasis Rev. 1985. PMID: 3893683 Review.
Do cells show an inverse locomotory response to fibronectin and laminin substrates?
Goodman SL, Newgreen D. Goodman SL, et al. EMBO J. 1985 Nov;4(11):2769-71. doi: 10.1002/j.1460-2075.1985.tb04002.x. EMBO J. 1985. PMID: 4065092 Free PMC article.
Signal transduction for chemotaxis and haptotaxis by matrix molecules in tumor cells.
Aznavoorian S, Stracke ML, Krutzsch H, Schiffmann E, Liotta LA. Aznavoorian S, et al. J Cell Biol. 1990 Apr;110(4):1427-38. doi: 10.1083/jcb.110.4.1427. J Cell Biol. 1990. PMID: 2324200 Free PMC article.
Morphological and metastatic murine melanoma variants: motility, adhesiveness, cell surface and in vivo properties.
Clark SR, Brody JS, Sidebottom E. Clark SR, et al. Br J Cancer. 1987 Nov;56(5):577-84. doi: 10.1038/bjc.1987.244. Br J Cancer. 1987. PMID: 3426920 Free PMC article.

References

1. Nature. 1967 Jan 21;213(5073):256-60 - PubMed
1. J Exp Med. 1968 Apr 1;127(4):693-709 - PubMed
1. J Biol Chem. 1970 Nov 10;245(21):5728-36 - PubMed
1. Int J Cancer. 1971 Jan 15;7(1):93-100 - PubMed
1. J Exp Med. 1972 Feb 1;135(2):376-87 - PubMed

Laminin and fibronectin promote the haptotactic migration of B16 mouse melanoma cells in vitro - PubMed (original) (raw)