Multiple immunoglobulin heavy-chain gene transcripts in Abelson murine leukemia virus-transformed lymphoid cell lines - PubMed (original) (raw)

Multiple immunoglobulin heavy-chain gene transcripts in Abelson murine leukemia virus-transformed lymphoid cell lines

F W Alt et al. Mol Cell Biol. 1982 Apr.

Abstract

Lymphoid cells transformed by Abelson murine leukemia virus (A-MuLV) contain three classes of RNA transcripts from immunoglobulin mu genes. P mu-mRNAs (productive) correspond to the normal 2.7-kilobase (kb) membrane (mu m) and 2.4-kb secreted (mu s) mu mRNA species both in size and coding capacity and occur at approximately equal abundance in most mu-positive (pre-B-like) A-MuLV transformants. A mu-mRNAs (aberrant) generally fall into one of two categories--aberrantly small 2.3-kb mu m and 2.0-kb mu s mRNAs which encode aberrantly small mu polypeptide chains, or normal-sized, V H-containing mu RNAs which do not encode immunologically identifiable mu polypeptide chains. In one case, the latter type of A mu-mRNA was demonstrated to result from an in-phase termination codon in the D segment of the mu mRNA. Also, most, if not all, A-MuLV transformants express members of a 3.0 to 1.9-kb set of C mu-containing, but V H-negative S mu-RNAs (for sterile), the expression of which may occur simultaneously with but independently of P mu-mRNAs or A mu-mRNAs. The S mu-RNA sequences do not encode immunologically identifiable mu chains and can be produced by cells with unrearranged heavy-chain alleles, such as T-lymphocytes, although the structure of the S mu-RNAs from T-lymphoid cells appears to be different from that of B-lymphoid cell S mu-RNAs. Certain A-MuLV transformants also express gamma-RNA sequences that are probably analogous to the three different forms of mu RNA. These data support the concept that heavy-chain allelic exclusion, like that of light chains, is not mediated by control at the DNA or RNA levels but is probably a consequence of feedback control from cytoplasmic mu chains.

PubMed Disclaimer

Cited by

Fine specificity, idiotypy, and nature of cloned heavy-chain variable region genes of murine monoclonal rheumatoid factor antibodies.
Manheimer-Lory AJ, Monestier M, Bellon B, Alt FW, Bona CA. Manheimer-Lory AJ, et al. Proc Natl Acad Sci U S A. 1986 Nov;83(21):8293-7. doi: 10.1073/pnas.83.21.8293. Proc Natl Acad Sci U S A. 1986. PMID: 3095832 Free PMC article.
Evidence of functional lymphocytes in some (leaky) scid mice.
Bosma GC, Fried M, Custer RP, Carroll A, Gibson DM, Bosma MJ. Bosma GC, et al. J Exp Med. 1988 Mar 1;167(3):1016-33. doi: 10.1084/jem.167.3.1016. J Exp Med. 1988. PMID: 3280724 Free PMC article.
Presence of a c-myc transcript initiated in intron 1 in Friend erythroleukemia cells and in other murine cell types with no evidence of c-myc gene rearrangement.
Ray D, Meneceur P, Tavitian A, Robert-Lezenes J. Ray D, et al. Mol Cell Biol. 1987 Feb;7(2):940-5. doi: 10.1128/mcb.7.2.940-945.1987. Mol Cell Biol. 1987. PMID: 3547087 Free PMC article.
From gene amplification to V(D)J recombination and back: a personal account of my early years in B cell biology.
Alt FW. Alt FW. Eur J Immunol. 2007 Nov;37 Suppl 1(Suppl 1):S138-47. doi: 10.1002/eji.200737848. Eur J Immunol. 2007. PMID: 17972338 Free PMC article.

References

1. Transplant Rev. 1975;25:26-58 - PubMed
1. J Exp Med. 1976 Jun 1;143(6):1453-63 - PubMed
1. Eur J Biochem. 1976 AUG 1;67(1):247-56 - PubMed
1. J Immunol. 1977 Apr;118(4):1181-90 - PubMed
1. Science. 1977 Apr 8;196(4286):161-9 - PubMed

Multiple immunoglobulin heavy-chain gene transcripts in Abelson murine leukemia virus-transformed lymphoid cell lines - PubMed (original) (raw)