Mutagenic deamination of cytosine residues in DNA - PubMed (original) (raw)

. 1980 Oct 9;287(5782):560-1.

doi: 10.1038/287560a0.

• PMID: 6999365
• DOI: 10.1038/287560a0

Mutagenic deamination of cytosine residues in DNA

B K Duncan et al. Nature. 1980.

Abstract

Spontaneous deamination converts cytosine to uracil, which is excised from DNA by the enzyme uracil-DNA glycosylase, leading to error-free repair. 5-Methylcytosine residues are deaminated to thymine, which cannot be excised and repaired by this system. As a result, 5-methylcytosine residues are hotspots for spontaneous transitions, as demonstrated in the lacI gene of Escherichia coli. We show here that in bacteria which lack uracil-DNA glycosylase (Ung-) and cannot excise uracil residues from DNA, the rate of spontaneous transition at cytosine residues is raised to the hotspot rate at 5-methylcytosine residues. These studies provide direct evidence that the deamination of cytosine is a significant source of spontaneous mutations.

PubMed Disclaimer

Similar articles

• Differential enhancement of spontaneous transition mutations in the lacI gene of an Ung- strain of Escherichia coli.
  Fix DF, Glickman BW. Fix DF, et al. Mutat Res. 1986 Oct;175(2):41-5. doi: 10.1016/0165-7992(86)90123-5. Mutat Res. 1986. PMID: 3531843
• Specific mutator effects of ung (uracil-DNA glycosylase) mutations in Escherichia coli.
  Duncan BK, Weiss B. Duncan BK, et al. J Bacteriol. 1982 Aug;151(2):750-5. doi: 10.1128/jb.151.2.750-755.1982. J Bacteriol. 1982. PMID: 7047496 Free PMC article.
• Accelerated deamination of cytosine residues in UV-induced cyclobutane pyrimidine dimers leads to CC-->TT transitions.
  Peng W, Shaw BR. Peng W, et al. Biochemistry. 1996 Aug 6;35(31):10172-81. doi: 10.1021/bi960001x. Biochemistry. 1996. PMID: 8756482
• Structure and function in the uracil-DNA glycosylase superfamily.
  Pearl LH. Pearl LH. Mutat Res. 2000 Aug 30;460(3-4):165-81. doi: 10.1016/s0921-8777(00)00025-2. Mutat Res. 2000. PMID: 10946227 Review.
• Properties and functions of human uracil-DNA glycosylase from the UNG gene.
  Krokan HE, Otterlei M, Nilsen H, Kavli B, Skorpen F, Andersen S, Skjelbred C, Akbari M, Aas PA, Slupphaug G. Krokan HE, et al. Prog Nucleic Acid Res Mol Biol. 2001;68:365-86. doi: 10.1016/s0079-6603(01)68112-1. Prog Nucleic Acid Res Mol Biol. 2001. PMID: 11554311 Review.

Cited by

• PRC2-AgeIndex as a universal biomarker of aging and rejuvenation.
  Moqri M, Cipriano A, Simpson DJ, Rasouli S, Murty T, de Jong TA, Nachun D, de Sena Brandine G, Ying K, Tarkhov A, Aberg KA, van den Oord E, Zhou W, Smith A, Mackall C, Gladyshev VN, Horvath S, Snyder MP, Sebastiano V. Moqri M, et al. Nat Commun. 2024 Jul 16;15(1):5956. doi: 10.1038/s41467-024-50098-2. Nat Commun. 2024. PMID: 39009581 Free PMC article.
• Metagenomic clustering links specific metabolic functions to globally relevant ecosystems.
  Flinkstrom Z, Bryson S, Candry P, Winkler M-KH. Flinkstrom Z, et al. mSystems. 2024 Aug 20;9(8):e0057324. doi: 10.1128/msystems.00573-24. Epub 2024 Jul 9. mSystems. 2024. PMID: 38980052 Free PMC article.
• Pan-cancer analysis of the interplay between mutational signatures and cellular signaling.
  Hakobyan A, Meyenberg M, Vardazaryan N, Hancock J, Vulliard L, Loizou JI, Menche J. Hakobyan A, et al. iScience. 2024 May 2;27(6):109873. doi: 10.1016/j.isci.2024.109873. eCollection 2024 Jun 21. iScience. 2024. PMID: 38783997 Free PMC article.
• Characterization of the genetic determinants of context-specific DNA methylation in primary monocytes.
  Gilchrist JJ, Fang H, Danielli S, Tomkova M, Nassiri I, Ng E, Tong O, Taylor C, Muldoon D, Cohen LRZ, Al-Mossawi H, Lau E, Neville M, Schuster-Boeckler B, Knight JC, Fairfax BP. Gilchrist JJ, et al. Cell Genom. 2024 May 8;4(5):100541. doi: 10.1016/j.xgen.2024.100541. Epub 2024 Apr 24. Cell Genom. 2024. PMID: 38663408 Free PMC article.
• Elevated incidence of somatic mutations at prevalent genetic sites.
  Wang M, Li SC, Shen B. Wang M, et al. Brief Bioinform. 2024 Jan 22;25(2):bbae065. doi: 10.1093/bib/bbae065. Brief Bioinform. 2024. PMID: 38426321 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Nature Publishing Group

• Other Literature Sources

  • The Lens - Patent Citations

• Molecular Biology Databases

  • BioCyc

• Research Materials

  • NCI CPTC Antibody Characterization Program

• Miscellaneous

  • NCI CPTAC Assay Portal