Oxygen affinity and Bohr coefficients of dog blood - PubMed (original) (raw)
Oxygen affinity and Bohr coefficients of dog blood
R B Reeves et al. J Appl Physiol Respir Environ Exerc Physiol. 1982 Jul.
Abstract
Complete dynamic oxygen equilibrium curves (O2EC) on dog whole blood were measured at 25 and 39 degrees C using a spectrophotometric micro blood film technique. O2EC were run at three CO2 levels (2, 4, and 8%) for each of three base excess levels (-10, 0, +10 meq/l). The standard curve (ph 7.4) was determined for saturations 0-0.98. At 39 degrees C the standard curve O2 pressure at half-saturation (P50) was 31.5 Torr; fixed-acid Bohr factor, -0.488; CO2 Bohr factor, -0.498; delta log P50/delta log PCO2, -0.0045. CO2 Bohr slope was linear over the pH range of 7-8. Bohr factors were not significantly saturation dependent. At 25 degrees C P50 was 15.4 Torr and CO2 Bohr factor, -0.647. The temperature coefficient (delta log P50/delta T) equaled 0.022. Dog O2EC were shown with curve-fitting techniques to be isomorphic with human blood O2EC. The absence of significant oxylabile carbamate formation in dog red blood cells (RBC) was attributed to high 2,3-diphosphoglycerate (DPG) concentrations, 6.23 mM/l RBC, equal to a DPG/Hb4 ratio of 1.12. A simple two-constant equation S = [(37,900)/(P3 + 205P) + 1]-1, where S is saturation and P is oxygen tension, was found to fit the dog 39 degrees C standard curve.
Similar articles
- Bohr effect data for blood gas calculations.
Hlastala MP, Woodson RD. Hlastala MP, et al. J Appl Physiol Respir Environ Exerc Physiol. 1983 Sep;55(3):1002-7. doi: 10.1152/jappl.1983.55.3.1002. J Appl Physiol Respir Environ Exerc Physiol. 1983. PMID: 6415006 - Determination of interdependent ligand effects on human red cell oxygen affinity.
Zijlstra WG, Oeseburg B, Kwant G, Zwart A. Zijlstra WG, et al. Scand J Clin Lab Invest. 1982 Jun;42(4):339-45. Scand J Clin Lab Invest. 1982. PMID: 6813948 - Red blood cell pH, the Bohr effect, and other oxygenation-linked phenomena in blood O2 and CO2 transport.
Jensen FB. Jensen FB. Acta Physiol Scand. 2004 Nov;182(3):215-27. doi: 10.1111/j.1365-201X.2004.01361.x. Acta Physiol Scand. 2004. PMID: 15491402 Review. - The magnitude of the Bohr effect profoundly influences the shape and position of the blood oxygen equilibrium curve.
Malte H, Lykkeboe G, Wang T. Malte H, et al. Comp Biochem Physiol A Mol Integr Physiol. 2021 Apr;254:110880. doi: 10.1016/j.cbpa.2020.110880. Epub 2020 Dec 20. Comp Biochem Physiol A Mol Integr Physiol. 2021. PMID: 33358924 Review.
Cited by
- Open-loop (feed-forward) and feedback control of coronary blood flow during exercise, cardiac pacing, and pressure changes.
Pradhan RK, Feigl EO, Gorman MW, Brengelmann GL, Beard DA. Pradhan RK, et al. Am J Physiol Heart Circ Physiol. 2016 Jun 1;310(11):H1683-94. doi: 10.1152/ajpheart.00663.2015. Epub 2016 Apr 1. Am J Physiol Heart Circ Physiol. 2016. PMID: 27037372 Free PMC article. - Adenine nucleotide control of coronary blood flow during exercise.
Gorman MW, Rooke GA, Savage MV, Jayasekara MP, Jacobson KA, Feigl EO. Gorman MW, et al. Am J Physiol Heart Circ Physiol. 2010 Dec;299(6):H1981-9. doi: 10.1152/ajpheart.00611.2010. Epub 2010 Sep 17. Am J Physiol Heart Circ Physiol. 2010. PMID: 20852039 Free PMC article. - Validation of oxygen extraction fraction measurement by qBOLD technique.
He X, Zhu M, Yablonskiy DA. He X, et al. Magn Reson Med. 2008 Oct;60(4):882-8. doi: 10.1002/mrm.21719. Magn Reson Med. 2008. PMID: 18816808 Free PMC article. - Modulation of erythropoietin formation by changes in blood volume in conscious dogs.
Ehmke H, Just A, Eckardt KU, Persson PB, Bauer C, Kirchheim HR. Ehmke H, et al. J Physiol. 1995 Oct 1;488 ( Pt 1)(Pt 1):181-91. doi: 10.1113/jphysiol.1995.sp020956. J Physiol. 1995. PMID: 8568654 Free PMC article. - Endogenous adenosine mediates coronary vasodilation during exercise after K(ATP)+ channel blockade.
Duncker DJ, van Zon NS, Pavek TJ, Herrlinger SK, Bache RJ. Duncker DJ, et al. J Clin Invest. 1995 Jan;95(1):285-95. doi: 10.1172/JCI117653. J Clin Invest. 1995. PMID: 7814627 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Research Materials