A novel enzymatic pathway leading to 1-methylinosine modification in Haloferax volcanii tRNA - PubMed (original) (raw)
A novel enzymatic pathway leading to 1-methylinosine modification in Haloferax volcanii tRNA
H Grosjean et al. Nucleic Acids Res. 1995.
Free PMC article
Abstract
Transfer RNAs of the extreme halophile Haloferax volcanii contain several modified nucleosides, among them 1-methylpseudouridine (m1 psi), pseudouridine (psi), 2'-0-methylcytosine (Cm) and 1-methylinosine (m1l), present in positions 54, 55, 56 and 57 of the psi-loop, respectively. At the same positions in tRNAs from eubacteria and eukaryotes, ribothymidine (T-54), pseudouridine (psi-55), non-modified cytosine (C-56) and non-modified adenosine or guanosine (A-57 or G-57) are found in the so-called T psi-loop. Using as substrate a T7 transcript of Haloferax volcanii tRNA(Ile) devoid of modified nucleosides, the enzymatic activities of several tRNA modification enzymes, including those for m1 psi-54, psi-55, Cm-56 and m1l-57, were detected in cell extracts of H.volcanii. Here, we demonstrate that modification of A-57 into m1l-57 in H.volcanii tRNA(Ile) occurs via a two-step enzymatic process. The first step corresponds to the formation of m1A-57 catalyzed by a S-adenosylmethionine-dependent tRNA methyltransferase, followed by the deamination of the 6-amino group of the adenine moiety by a 1-methyladenosine-57 deaminase. This enzymatic pathway differs from that leading to the formation of m1l-37 in the anticodon loop of eukaryotic tRNA(Ala). In the latter case, inosine-37 formation preceeds the S-adenosylmethionine-dependent methylation of l-37 into m1l-37. Thus, enzymatic strategies for catalyzing the formation of 1-methylinosine in tRNAs differ in organisms from distinct evolutionary kingdoms.
Similar articles
- Enzymatic conversion of adenosine to inosine and to N1-methylinosine in transfer RNAs: a review.
Grosjean H, Auxilien S, Constantinesco F, Simon C, Corda Y, Becker HF, Foiret D, Morin A, Jin YX, Fournier M, Fourrey JL. Grosjean H, et al. Biochimie. 1996;78(6):488-501. doi: 10.1016/0300-9084(96)84755-9. Biochimie. 1996. PMID: 8915538 Review. - Major identity determinants for enzymatic formation of ribothymidine and pseudouridine in the T psi-loop of yeast tRNAs.
Becker HF, Motorin Y, Sissler M, Florentz C, Grosjean H. Becker HF, et al. J Mol Biol. 1997 Dec 12;274(4):505-18. doi: 10.1006/jmbi.1997.1417. J Mol Biol. 1997. PMID: 9417931 - Intron-dependent formation of pseudouridines in the anticodon of Saccharomyces cerevisiae minor tRNA(Ile).
Szweykowska-Kulinska Z, Senger B, Keith G, Fasiolo F, Grosjean H. Szweykowska-Kulinska Z, et al. EMBO J. 1994 Oct 3;13(19):4636-44. doi: 10.1002/j.1460-2075.1994.tb06786.x. EMBO J. 1994. PMID: 7925304 Free PMC article. - The modified wobble base inosine in yeast tRNAIle is a positive determinant for aminoacylation by isoleucyl-tRNA synthetase.
Senger B, Auxilien S, Englisch U, Cramer F, Fasiolo F. Senger B, et al. Biochemistry. 1997 Jul 8;36(27):8269-75. doi: 10.1021/bi970206l. Biochemistry. 1997. PMID: 9204872 - Pseudouridine: still mysterious, but never a fake (uridine)!
Spenkuch F, Motorin Y, Helm M. Spenkuch F, et al. RNA Biol. 2014;11(12):1540-54. doi: 10.4161/15476286.2014.992278. RNA Biol. 2014. PMID: 25616362 Free PMC article. Review.
Cited by
- The occurrence, characteristics, and adaptation of A-to-I RNA editing in bacteria: A review.
Liao W, Nie W, Ahmad I, Chen G, Zhu B. Liao W, et al. Front Microbiol. 2023 Mar 7;14:1143929. doi: 10.3389/fmicb.2023.1143929. eCollection 2023. Front Microbiol. 2023. PMID: 36960293 Free PMC article. Review. - Modifications of the human tRNA anticodon loop and their associations with genetic diseases.
Zhou JB, Wang ED, Zhou XL. Zhou JB, et al. Cell Mol Life Sci. 2021 Dec;78(23):7087-7105. doi: 10.1007/s00018-021-03948-x. Epub 2021 Oct 4. Cell Mol Life Sci. 2021. PMID: 34605973 Free PMC article. Review. - Inosine in Biology and Disease.
Srinivasan S, Torres AG, Ribas de Pouplana L. Srinivasan S, et al. Genes (Basel). 2021 Apr 19;12(4):600. doi: 10.3390/genes12040600. Genes (Basel). 2021. PMID: 33921764 Free PMC article. Review. - Post-Transcriptional Modifications of Conserved Nucleotides in the T-Loop of tRNA: A Tale of Functional Convergent Evolution.
Roovers M, Droogmans L, Grosjean H. Roovers M, et al. Genes (Basel). 2021 Jan 22;12(2):140. doi: 10.3390/genes12020140. Genes (Basel). 2021. PMID: 33499018 Free PMC article. Review. - Comparative patterns of modified nucleotides in individual tRNA species from a mesophilic and two thermophilic archaea.
Wolff P, Villette C, Zumsteg J, Heintz D, Antoine L, Chane-Woon-Ming B, Droogmans L, Grosjean H, Westhof E. Wolff P, et al. RNA. 2020 Dec;26(12):1957-1975. doi: 10.1261/rna.077537.120. Epub 2020 Sep 29. RNA. 2020. PMID: 32994183 Free PMC article.
References
- J Biol Chem. 1982 Apr 10;257(7):3589-92 - PubMed
- J Bacteriol. 1978 Jan;133(1):240-50 - PubMed
- Nucleic Acids Symp Ser. 1982;(11):209-13 - PubMed
- Nucleic Acids Res. 1983 Aug 25;11(16):5433-42 - PubMed
- J Biol Chem. 1984 Aug 10;259(15):9461-71 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Research Materials