Failure to detect an antimutator phenotype following disruption of the Saccharomyces cerevisiae DDR48 gene - PubMed (original) (raw)

. 1995 May;27(6):496-500.

doi: 10.1007/BF00314438.

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Failure to detect an antimutator phenotype following disruption of the Saccharomyces cerevisiae DDR48 gene

H Roche et al. Curr Genet. 1995 May.

Abstract

The antimutator phenotype, reportedly conferred by disruption of the Saccharomyces cerevisiae DDR48 gene, was suggested to affect only a specific spontaneous mutational pathway. We attempted to identify the types of mutation that are DDR48-dependent by determining the specificity of the ddr48 antimutator. However, disruption of DDR48 did not decrease the rates of spontaneous forward mutation in a plasmid-borne copy of the yeast SUP4-o gene, the reversion or suppression of the lys2-1 allele, or forward mutation at the CAN1 locus. Interestingly, the latter gene had been reported previously to be subject to the antimutator effect. DNA sequence analysis of spontaneous SUP4-o mutations arising in DDR48 and ddr48 backgrounds provided no evidence for a reduction in the rates of individual mutational classes. Thus, we were unable to verify that disruption of DDR48 causes an antimutator phenotype.

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