Heparin is an adhesive ligand for the leukocyte integrin Mac-1 (CD11b/CD1) - PubMed (original) (raw)

Heparin is an adhesive ligand for the leukocyte integrin Mac-1 (CD11b/CD1)

M S Diamond et al. J Cell Biol. 1995 Sep.

Abstract

Previous studies have demonstrated that the leukocyte integrin Mac-1 adheres to several cell surface and soluble ligands including intercellular adhesion molecule-1, fibrinogen, iC3b, and factor X. However, experiments with Mac-1-expressing transfectants, purified Mac-1, and mAbs to Mac-1 indicate the existence of additional ligands. In this paper, we demonstrate a direct interaction between Mac-1 and heparan sulfate glycans. Heparin affinity resins immunoprecipitate Mac-1, and neutrophils and transfectant cells that express Mac-1 bind to heparin and heparan sulfate, but not to other sulfated glycosaminoglycans. Inhibition studies with mAbs and chemically modified forms of heparin suggest the I domain as a recognition site on Mac-1 for heparin, and suggest that either N- or O-sulfation is sufficient for heparin to bind efficiently to Mac-1. Under conditions of continuous flow in which heparins and E-selectin are cosubstrates, neutrophils tether to E-selectin and form firm adhesions through a Mac-1-heparin interaction.

PubMed Disclaimer

Similar articles

Cited by

References

    1. J Biol Chem. 1994 Jun 24;269(25):17075-9 - PubMed
    1. J Biol Chem. 1994 Jul 22;269(29):18881-90 - PubMed
    1. Blood. 1994 Aug 1;84(3):739-52 - PubMed
    1. Proc Natl Acad Sci U S A. 1994 Oct 25;91(22):10680-4 - PubMed
    1. Proc Natl Acad Sci U S A. 1994 Nov 8;91(23):11035-9 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources