Exit from mitosis is regulated by Drosophila fizzy and the sequential destruction of cyclins A, B and B3 - PubMed (original) (raw)
Exit from mitosis is regulated by Drosophila fizzy and the sequential destruction of cyclins A, B and B3
S Sigrist et al. EMBO J. 1995.
Abstract
While entry into mitosis is triggered by activation of cdc2 kinase, exit from mitosis requires inactivation of this kinase. Inactivation results from proteolytic degradation of the regulatory cyclin subunits during mitosis. At least three different cyclin types, cyclins A, B and B3, associate with cdc2 kinase in higher eukaryotes and are sequentially degraded in mitosis. We show here that mutations in the Drosophila gene fizzy (fzy) block the mitotic degradation of these cyclins. Moreover, expression of mutant cyclins (delta cyclins) lacking the destruction box motif required for mitotic degradation affects mitotic progression at distinct stages. Deltacyclin A results in a delay in metaphase, deltacyclin B in an early anaphase arrest and deltacyclin B3 in a late anaphase arrest, suggesting that mitotic progression beyond metaphase is ordered by the sequential degradation of these different cyclins. Coexpression of deltacyclins A, B and B3 allows a delayed separation of sister chromosomes, but interferes wit chromosome segregation to the poles. Mutations in fzy block both sister chromosome separation and segregation, indicating that fzy plays a crucial role in the metaphase/anaphase transition.
Similar articles
- The Drosophila cell cycle gene fizzy is required for normal degradation of cyclins A and B during mitosis and has homology to the CDC20 gene of Saccharomyces cerevisiae.
Dawson IA, Roth S, Artavanis-Tsakonas S. Dawson IA, et al. J Cell Biol. 1995 May;129(3):725-37. doi: 10.1083/jcb.129.3.725. J Cell Biol. 1995. PMID: 7730407 Free PMC article. - The schedule of destruction of three mitotic cyclins can dictate the timing of events during exit from mitosis.
Parry DH, O'Farrell PH. Parry DH, et al. Curr Biol. 2001 May 1;11(9):671-83. doi: 10.1016/s0960-9822(01)00204-4. Curr Biol. 2001. PMID: 11369230 Free PMC article. - Pds1 and Esp1 control both anaphase and mitotic exit in normal cells and after DNA damage.
Tinker-Kulberg RL, Morgan DO. Tinker-Kulberg RL, et al. Genes Dev. 1999 Aug 1;13(15):1936-49. doi: 10.1101/gad.13.15.1936. Genes Dev. 1999. PMID: 10444592 Free PMC article. - Cyclin destruction in mitosis: a crucial task of Cdc20.
Irniger S. Irniger S. FEBS Lett. 2002 Dec 4;532(1-2):7-11. doi: 10.1016/s0014-5793(02)03657-8. FEBS Lett. 2002. PMID: 12459453 Review. - '... The end of the beginning': cdk1 thresholds and exit from mitosis.
Wolf F, Sigl R, Geley S. Wolf F, et al. Cell Cycle. 2007 Jun 15;6(12):1408-11. Epub 2007 Apr 27. Cell Cycle. 2007. PMID: 17581279 Review.
Cited by
- Cyclin B3 is a dominant fast-acting cyclin that drives rapid early embryonic mitoses.
Lara-Gonzalez P, Variyar S, Moghareh S, Nguyen ACN, Kizhedathu A, Budrewicz J, Schlientz A, Varshney N, Bellaart A, Oegema K, Bardwell L, Desai A. Lara-Gonzalez P, et al. J Cell Biol. 2024 Nov 4;223(11):e202308034. doi: 10.1083/jcb.202308034. Epub 2024 Aug 6. J Cell Biol. 2024. PMID: 39105756 - Analog-sensitive Cdk1 as a tool to study mitotic exit: protein phosphatase 1 is required downstream from Cdk1 inactivation in budding yeast.
Keaton JM, Workman BG, Xie L, Paulson JR. Keaton JM, et al. Chromosome Res. 2023 Sep 10;31(3):27. doi: 10.1007/s10577-023-09736-6. Chromosome Res. 2023. PMID: 37690059 - Meiotic Cell Cycle Progression in Mouse Oocytes: Role of Cyclins.
Kim HM, Kang MK, Seong SY, Jo JH, Kim MJ, Shin EK, Lee CG, Han SJ. Kim HM, et al. Int J Mol Sci. 2023 Sep 4;24(17):13659. doi: 10.3390/ijms241713659. Int J Mol Sci. 2023. PMID: 37686466 Free PMC article. Review. - RNAi Analysis of Potential Functions of Cyclin B3 in Reproduction of Male Oriental River Prawns (Macrobrachium nipponense).
Jin S, Zhou Z, Zhang W, Xiong Y, Qiao H, Gong Y, Wu Y, Jiang S, Fu H. Jin S, et al. Animals (Basel). 2023 May 21;13(10):1703. doi: 10.3390/ani13101703. Animals (Basel). 2023. PMID: 37238135 Free PMC article. - Exit from Mitosis in Budding Yeast: Protein Phosphatase 1 is Required Downstream from Cdk1 Inactivation.
Keaton JM, Workman BG, Xie L, Paulson JR. Keaton JM, et al. Res Sq [Preprint]. 2023 Apr 12:rs.3.rs-2787001. doi: 10.21203/rs.3.rs-2787001/v1. Res Sq. 2023. PMID: 37090579 Free PMC article. Updated. Preprint.
References
- EMBO J. 1994 Feb 1;13(3):595-605 - PubMed
- EMBO J. 1990 Aug;9(8):2563-72 - PubMed
- Cell. 1993 Jul 2;73(7):1393-402 - PubMed
- Cell. 1990 May 4;61(3):535-47 - PubMed
- Nature. 1995 Jan 5;373(6509):78-81 - PubMed
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases
Miscellaneous