A Drosophila shc gene product is implicated in signaling by the DER receptor tyrosine kinase - PubMed (original) (raw)
A Drosophila shc gene product is implicated in signaling by the DER receptor tyrosine kinase
K M Lai et al. Mol Cell Biol. 1995 Sep.
Abstract
Antibodies to the human Shc adaptor protein were used to isolate a cDNA encoding a Drosophila Shc protein (dShc) by screening an expression library. The dshc gene, which maps to position 67B-C on the third chromosome, encodes a 45-kDa protein that is widely expressed throughout the Drosophila life cycle. In flies, the dShc protein physically associates with the activated Drosophila epidermal growth factor receptor homolog (DER) and is inducibly phosphorylated on tyrosine by DER. The 45-kDa dShc protein is closely related both in overall organization and in amino acid sequence (46% identity) to the 52-kDa mammalian Shc isoform. In addition to a C-terminal Src homology 2 (SH2) domain, dShc contains an N-terminal phosphotyrosine-binding (PTB) domain, which associates in vitro with the autophosphorylated DER receptor tyrosine kinase and with phosphopeptides containing an Asn-Pro-X-pTyr motif, where pTyr stands for phosphotyrosine. A potential binding site for the dShc PTB domain is located at Tyr-1228 of DER. These results indicate that the shc gene has been conserved in evolution, as have the binding properties of the Shc PTB and SH2 domains. Despite the close relationship between the Drosophila and mammalian Shc proteins, dShc lacks the high-affinity Grb2-binding site found in mammalian Shc, suggesting that Shc proteins may have functions in addition to regulation of the Ras pathway.
Similar articles
- Characterization of the phosphotyrosine-binding domain of the Drosophila Shc protein.
Li SC, Lai KM, Gish GD, Parris WE, van der Geer P, Forman-Kay J, Pawson T. Li SC, et al. J Biol Chem. 1996 Dec 13;271(50):31855-62. doi: 10.1074/jbc.271.50.31855. J Biol Chem. 1996. PMID: 8943228 - A conserved amino-terminal Shc domain binds to phosphotyrosine motifs in activated receptors and phosphopeptides.
van der Geer P, Wiley S, Lai VK, Olivier JP, Gish GD, Stephens R, Kaplan D, Shoelson S, Pawson T. van der Geer P, et al. Curr Biol. 1995 Apr 1;5(4):404-12. doi: 10.1016/s0960-9822(95)00081-9. Curr Biol. 1995. PMID: 7542991 - The Drosophila SHC adaptor protein is required for signaling by a subset of receptor tyrosine kinases.
Luschnig S, Krauss J, Bohmann K, Desjeux I, Nüsslein-Volhard C. Luschnig S, et al. Mol Cell. 2000 Feb;5(2):231-41. doi: 10.1016/s1097-2765(00)80419-0. Mol Cell. 2000. PMID: 10882065 - Evolution of Shc functions from nematode to human.
Luzi L, Confalonieri S, Di Fiore PP, Pelicci PG. Luzi L, et al. Curr Opin Genet Dev. 2000 Dec;10(6):668-74. doi: 10.1016/s0959-437x(00)00146-5. Curr Opin Genet Dev. 2000. PMID: 11088019 Review. - Emerging roles for SH2/PTB-containing Shc adaptor proteins in the developing mammalian brain.
Cattaneo E, Pelicci PG. Cattaneo E, et al. Trends Neurosci. 1998 Nov;21(11):476-81. doi: 10.1016/s0166-2236(98)01282-x. Trends Neurosci. 1998. PMID: 9829689 Review.
Cited by
- Endocytosis at the Crossroad of Polarity and Signaling Regulation: Learning from Drosophila melanogaster and Beyond.
Papagiannouli F. Papagiannouli F. Int J Mol Sci. 2022 Apr 23;23(9):4684. doi: 10.3390/ijms23094684. Int J Mol Sci. 2022. PMID: 35563080 Free PMC article. Review. - Comparative transcriptome analyses of the Drosophila pupal eye.
DeAngelis MW, Coolon JD, Johnson RI. DeAngelis MW, et al. G3 (Bethesda). 2021 Jan 18;11(1):jkaa003. doi: 10.1093/g3journal/jkaa003. G3 (Bethesda). 2021. PMID: 33561221 Free PMC article. - Regulatory mechanisms of EGFR signalling during Drosophila eye development.
Malartre M. Malartre M. Cell Mol Life Sci. 2016 May;73(9):1825-43. doi: 10.1007/s00018-016-2153-x. Epub 2016 Mar 2. Cell Mol Life Sci. 2016. PMID: 26935860 Free PMC article. Review. - ShcA Protects against Epithelial-Mesenchymal Transition through Compartmentalized Inhibition of TGF-β-Induced Smad Activation.
Muthusamy BP, Budi EH, Katsuno Y, Lee MK, Smith SM, Mirza AM, Akhurst RJ, Derynck R. Muthusamy BP, et al. PLoS Biol. 2015 Dec 17;13(12):e1002325. doi: 10.1371/journal.pbio.1002325. eCollection 2015 Dec. PLoS Biol. 2015. PMID: 26680585 Free PMC article. - The PTB domain of ShcA couples receptor activation to the cytoskeletal regulator IQGAP1.
Smith MJ, Hardy WR, Li GY, Goudreault M, Hersch S, Metalnikov P, Starostine A, Pawson T, Ikura M. Smith MJ, et al. EMBO J. 2010 Mar 3;29(5):884-96. doi: 10.1038/emboj.2009.399. Epub 2010 Jan 14. EMBO J. 2010. PMID: 20075861 Free PMC article.
References
- Nature. 1994 Jan 6;367(6458):87-90 - PubMed
- J Biol Chem. 1993 Aug 25;268(24):17659-61 - PubMed
- Genes Dev. 1994 Feb 15;8(4):428-39 - PubMed
- Mol Cell Biol. 1994 Apr;14(4):2777-85 - PubMed
- Neuron. 1994 Mar;12(3):691-705 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Research Materials
Miscellaneous