An unusual buried polar cluster in a family of fungal lipases - PubMed (original) (raw)
Comparative Study
doi: 10.1038/nsb0194-36.
Affiliations
- PMID: 7656005
- DOI: 10.1038/nsb0194-36
Comparative Study
An unusual buried polar cluster in a family of fungal lipases
U Derewenda et al. Nat Struct Biol. 1994 Jan.
Abstract
The stability of globular proteins arises largely from the burial of non-polar amino acids in their interior. These residues are efficiently packed to eliminate energetically unfavorable cavities. Contrary to these observations, high resolution X-ray crystallographic analyses of four homologous lipases from filamentous fungi reveal an alpha/beta fold which contains a buried conserved constellation of charged and polar side chains with associated cavities containing ordered water molecules. It is possible that this structural arrangement plays an important role in interfacial catalysis.
Similar articles
- Essential dynamics of lipase binding sites: the effect of inhibitors of different chain length.
Peters GH, van Aalten DM, Svendsen A, Bywater R. Peters GH, et al. Protein Eng. 1997 Feb;10(2):149-58. doi: 10.1093/protein/10.2.149. Protein Eng. 1997. PMID: 9089814 - Current progress in crystallographic studies of new lipases from filamentous fungi.
Derewenda U, Swenson L, Green R, Wei Y, Yamaguchi S, Joerger R, Haas MJ, Derewenda ZS. Derewenda U, et al. Protein Eng. 1994 Apr;7(4):551-7. doi: 10.1093/protein/7.4.551. Protein Eng. 1994. PMID: 8029211 - Computational studies of the activation of lipases and the effect of a hydrophobic environment.
Peters GH, Toxvaerd S, Olsen OH, Svendsen A. Peters GH, et al. Protein Eng. 1997 Feb;10(2):137-47. doi: 10.1093/protein/10.2.137. Protein Eng. 1997. PMID: 9089813 - Structural and evolutionary relationships in lipase mechanism and activation.
Dodson GG, Lawson DM, Winkler FK. Dodson GG, et al. Faraday Discuss. 1992;(93):95-105. doi: 10.1039/fd9929300095. Faraday Discuss. 1992. PMID: 1290943 Review. - Gastric lipase: crystal structure and activity.
Canaan S, Roussel A, Verger R, Cambillau C. Canaan S, et al. Biochim Biophys Acta. 1999 Nov 23;1441(2-3):197-204. doi: 10.1016/s1388-1981(99)00160-2. Biochim Biophys Acta. 1999. PMID: 10570247 Review.
Cited by
- Cultivation conditions and properties of extracellular crude lipase from the psychrotrophic fungus Penicillium chrysogenum 9'.
Bancerz R, Ginalska G, Fiedurek J, Gromada A. Bancerz R, et al. J Ind Microbiol Biotechnol. 2005 Jun;32(6):253-60. doi: 10.1007/s10295-005-0235-0. Epub 2005 May 14. J Ind Microbiol Biotechnol. 2005. PMID: 15895263 - Crystal structure of a secreted lipase from Gibberella zeae reveals a novel "double-lock" mechanism.
Lou Z, Li M, Sun Y, Liu Y, Liu Z, Wu W, Rao Z. Lou Z, et al. Protein Cell. 2010 Aug;1(8):760-70. doi: 10.1007/s13238-010-0094-y. Epub 2010 Aug 28. Protein Cell. 2010. PMID: 21203917 Free PMC article. - The Lid Domain in Lipases: Structural and Functional Determinant of Enzymatic Properties.
Khan FI, Lan D, Durrani R, Huan W, Zhao Z, Wang Y. Khan FI, et al. Front Bioeng Biotechnol. 2017 Mar 9;5:16. doi: 10.3389/fbioe.2017.00016. eCollection 2017. Front Bioeng Biotechnol. 2017. PMID: 28337436 Free PMC article. Review. - Malonyl-CoA: acyl carrier protein transacylase from Helicobacter pylori: Crystal structure and its interaction with acyl carrier protein.
Zhang L, Liu W, Xiao J, Hu T, Chen J, Chen K, Jiang H, Shen X. Zhang L, et al. Protein Sci. 2007 Jun;16(6):1184-92. doi: 10.1110/ps.072757307. Protein Sci. 2007. PMID: 17525466 Free PMC article. - Influence of a lipid interface on protein dynamics in a fungal lipase.
Peters GH, Bywater RP. Peters GH, et al. Biophys J. 2001 Dec;81(6):3052-65. doi: 10.1016/S0006-3495(01)75944-9. Biophys J. 2001. PMID: 11720974 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Molecular Biology Databases