Phosphoinositide 3-kinase as an upstream regulator of the small GTP-binding protein Rac in the insulin signaling of membrane ruffling - PubMed (original) (raw)
. 1995 Mar 28;208(3):985-90.
doi: 10.1006/bbrc.1995.1431.
Affiliations
- PMID: 7702629
- DOI: 10.1006/bbrc.1995.1431
Phosphoinositide 3-kinase as an upstream regulator of the small GTP-binding protein Rac in the insulin signaling of membrane ruffling
K Kotani et al. Biochem Biophys Res Commun. 1995.
Abstract
Membrane ruffling and the closely linked response of fluid-phase pinocytosis were investigated in Chinese hamster ovary cells that stably overexpress the human insulin receptor and a mutant 85-kDa subunit of phosphoinositide (PI) 3-kinase (delta p85) that lacks a binding site for the catalytic 110-kDa subunit of this enzyme. Both membrane ruffling and pinocytosis induced by insulin were markedly impaired in these cells. Microinjection of Rac, a Ras-related small GTP-binding protein, induced membrane ruffling in human epidermoid carcinoma KB cells, and this effect of Rac was not blocked by coinjection of delta p85 or by exposure of cells to wortmannin, a specific PI 3-kinase inhibitor. These results suggest that PI 3-kinase is essential not only for insulin-stimulated membrane ruffling but also for pinocytosis, and that PI 3-kinase possibly functions upstream of Rac in the signal transduction pathway.
Similar articles
- Role of PI 3-kinase and SH-PTP2 in insulin action.
Kasuga M. Kasuga M. Diabet Med. 1996 Sep;13(9 Suppl 6):S87-9. Diabet Med. 1996. PMID: 8894489 Review. - Membrane ruffling and signal transduction.
Ridley AJ. Ridley AJ. Bioessays. 1994 May;16(5):321-7. doi: 10.1002/bies.950160506. Bioessays. 1994. PMID: 8024541 Review. - EGF-induced activation of 70-kDa S6 kinase in CHO cells expressing human EGF receptors.
Kido Y, Okabayashi Y, Okutani T, Sugimoto Y, Sakaguchi K, Kasuga M. Kido Y, et al. Biochem Biophys Res Commun. 1995 Apr 6;209(1):131-8. doi: 10.1006/bbrc.1995.1480. Biochem Biophys Res Commun. 1995. PMID: 7726827 - Ras-independent and wortmannin-sensitive activation of glycogen synthase by insulin in Chinese hamster ovary cells.
Sakaue H, Hara K, Noguchi T, Matozaki T, Kotani K, Ogawa W, Yonezawa K, Waterfield MD, Kasuga M. Sakaue H, et al. J Biol Chem. 1995 May 12;270(19):11304-9. doi: 10.1074/jbc.270.19.11304. J Biol Chem. 1995. PMID: 7744767 - Uncoupling of membrane ruffling and pinocytosis during Ras signal transduction.
Li G, D'Souza-Schorey C, Barbieri MA, Cooper JA, Stahl PD. Li G, et al. J Biol Chem. 1997 Apr 18;272(16):10337-40. J Biol Chem. 1997. PMID: 9099668
Cited by
- Unconventional myosin Myo1c promotes membrane fusion in a regulated exocytic pathway.
Bose A, Robida S, Furcinitti PS, Chawla A, Fogarty K, Corvera S, Czech MP. Bose A, et al. Mol Cell Biol. 2004 Jun;24(12):5447-58. doi: 10.1128/MCB.24.12.5447-5458.2004. Mol Cell Biol. 2004. PMID: 15169906 Free PMC article. - Constitutive macropinocytosis in oncogene-transformed fibroblasts depends on sequential permanent activation of phosphoinositide 3-kinase and phospholipase C.
Amyere M, Payrastre B, Krause U, Van Der Smissen P, Veithen A, Courtoy PJ. Amyere M, et al. Mol Biol Cell. 2000 Oct;11(10):3453-67. doi: 10.1091/mbc.11.10.3453. Mol Biol Cell. 2000. PMID: 11029048 Free PMC article. - Wortmannin blocks goldfish retinal phosphatidylinositol 3-kinase and neurite outgrowth.
Lavie Y, Dybowski J, Agranoff BW. Lavie Y, et al. Neurochem Res. 1997 Apr;22(4):373-8. doi: 10.1023/a:1027391206791. Neurochem Res. 1997. PMID: 9130246
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials
Miscellaneous