Distinct biochemical characteristics of the two human profilin isoforms - PubMed (original) (raw)
Comparative Study
Distinct biochemical characteristics of the two human profilin isoforms
R Gieselmann et al. Eur J Biochem. 1995.
Free article
Abstract
The biochemical characteristics of a new human profilin isoform are described. We refer to this recently described isoform as profilin II (isoelectric point 5.9) in comparison to profilin I (pI 8.4). We expressed both isoforms in bacteria and compared their actin-binding properties, binding to poly(L-proline), affinities for phosphatidylinositol 4,5-bisphosphate [PtdIns(4,5)P2], and their effects on nucleotide exchange on actin. Profilin I and profilin II have similar affinities for PtdIns(4,5)P2 and poly(L-proline), and both accelerate nucleotide exchange on monomeric actin to the same extent. However, the affinity of profilin I for monomeric actin is about five times higher than the affinity of profilin II for actin. Potential structural differences of profilin I and profilin II that might explain the difference in actin binding are discussed.
Similar articles
- Purification and characterization of bovine profilin II. Actin, poly(L-proline) and inositolphospholipid binding.
Lambrechts A, van Damme J, Goethals M, Vandekerckhove J, Ampe C. Lambrechts A, et al. Eur J Biochem. 1995 May 15;230(1):281-6. Eur J Biochem. 1995. PMID: 7601111 - X-ray structure determination of human profilin II: A comparative structural analysis of human profilins.
Nodelman IM, Bowman GD, Lindberg U, Schutt CE. Nodelman IM, et al. J Mol Biol. 1999 Dec 17;294(5):1271-85. doi: 10.1006/jmbi.1999.3318. J Mol Biol. 1999. PMID: 10600384 - Mutational analysis of human profilin I reveals a second PI(4,5)-P2 binding site neighbouring the poly(L-proline) binding site.
Lambrechts A, Jonckheere V, Dewitte D, Vandekerckhove J, Ampe C. Lambrechts A, et al. BMC Biochem. 2002 May 28;3:12. doi: 10.1186/1471-2091-3-12. BMC Biochem. 2002. PMID: 12052260 Free PMC article. - Profilin: at the crossroads of signal transduction and the actin cytoskeleton.
Sohn RH, Goldschmidt-Clermont PJ. Sohn RH, et al. Bioessays. 1994 Jul;16(7):465-72. doi: 10.1002/bies.950160705. Bioessays. 1994. PMID: 7945274 Review. - The three faces of profilin.
Theriot JA, Mitchison TJ. Theriot JA, et al. Cell. 1993 Dec 3;75(5):835-8. doi: 10.1016/0092-8674(93)90527-w. Cell. 1993. PMID: 8252619 Review. No abstract available.
Cited by
- Proteomic Approaches and Potential Applications in Autosomal Dominant Polycystic Kidney Disease and Fabry Disease.
Rroji M, Figurek A, Spasovski G. Rroji M, et al. Diagnostics (Basel). 2023 Mar 17;13(6):1152. doi: 10.3390/diagnostics13061152. Diagnostics (Basel). 2023. PMID: 36980460 Free PMC article. Review. - Activated I-BAR IRSp53 clustering controls the formation of VASP-actin-based membrane protrusions.
Tsai FC, Henderson JM, Jarin Z, Kremneva E, Senju Y, Pernier J, Mikhajlov O, Manzi J, Kogan K, Le Clainche C, Voth GA, Lappalainen P, Bassereau P. Tsai FC, et al. Sci Adv. 2022 Oct 14;8(41):eabp8677. doi: 10.1126/sciadv.abp8677. Epub 2022 Oct 14. Sci Adv. 2022. PMID: 36240267 Free PMC article. - Structure of the Monkeypox virus profilin-like protein A42R reveals potential functional differences from cellular profilins.
Minasov G, Inniss NL, Shuvalova L, Anderson WF, Satchell KJF. Minasov G, et al. Acta Crystallogr F Struct Biol Commun. 2022 Oct 1;78(Pt 10):371-377. doi: 10.1107/S2053230X22009128. Epub 2022 Sep 26. Acta Crystallogr F Struct Biol Commun. 2022. PMID: 36189721 Free PMC article. - Profilin choreographs actin and microtubules in cells and cancer.
Pimm ML, Hotaling J, Henty-Ridilla JL. Pimm ML, et al. Int Rev Cell Mol Biol. 2020;355:155-204. doi: 10.1016/bs.ircmb.2020.05.005. Epub 2020 Jul 16. Int Rev Cell Mol Biol. 2020. PMID: 32859370 Free PMC article. - Modulation of formin processivity by profilin and mechanical tension.
Cao L, Kerleau M, Suzuki EL, Wioland H, Jouet S, Guichard B, Lenz M, Romet-Lemonne G, Jegou A. Cao L, et al. Elife. 2018 May 25;7:e34176. doi: 10.7554/eLife.34176. Elife. 2018. PMID: 29799413 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases
Research Materials
Miscellaneous