p53 binds single-stranded DNA ends through the C-terminal domain and internal DNA segments via the middle domain - PubMed (original) (raw)
p53 binds single-stranded DNA ends through the C-terminal domain and internal DNA segments via the middle domain
G Bakalkin et al. Nucleic Acids Res. 1995.
Free PMC article
Abstract
We have previously reported that wild-type p53 can bind single-stranded (ss) DNA ends and catalyze renaturation of ss complementary DNA molecules. Here we demonstrate that p53 can also bind to internal segments of ss DNA molecules via a binding site (internal DNA site) distinct from the binding site for DNA ends (DNA end site). Using p53 deletion mutants, the internal DNA site was mapped to the central region (residues 99-307), while the DNA end site was mapped to the C-terminal domain (residues 320-393) of the p53 protein. The internal DNA site can be activated by the binding of ss DNA ends to the DNA end site. The C-terminal domain alone was sufficient to catalyze DNA renaturation, although the central domain was also involved in promotion of renaturation by the full-length protein. Our results suggest that the interaction of the C-terminal tail of p53 with ss DNA ends generated by DNA damage in vivo may lead to activation of non-specific ss DNA binding by the central domain of p53.
Similar articles
- The single-stranded DNA end binding site of p53 coincides with the C-terminal regulatory region.
Selivanova G, Iotsova V, Kiseleva E, Ström M, Bakalkin G, Grafström RC, Wiman KG. Selivanova G, et al. Nucleic Acids Res. 1996 Sep 15;24(18):3560-7. doi: 10.1093/nar/24.18.3560. Nucleic Acids Res. 1996. PMID: 8836183 Free PMC article. - The C-terminal domain of p53 catalyzes DNA-renaturation and strand exchange toward annealing between intact ssDNAs and toward eliminating damaged ssDNA from duplex formation through preferential recognition of damaged DNA by a duocarmycin.
Yokote H, Nishio K, Arioka H, Kurokawa H, Fukuoka K, Fukumoto H, Ishida T, Terada T, Itakura T, Saijo N. Yokote H, et al. Mutat Res. 1998 Dec 14;409(3):147-62. doi: 10.1016/s0921-8777(98)00052-4. Mutat Res. 1998. PMID: 9875290 - p53 binds single-stranded DNA ends and catalyzes DNA renaturation and strand transfer.
Bakalkin G, Yakovleva T, Selivanova G, Magnusson KP, Szekely L, Kiseleva E, Klein G, Terenius L, Wiman KG. Bakalkin G, et al. Proc Natl Acad Sci U S A. 1994 Jan 4;91(1):413-7. doi: 10.1073/pnas.91.1.413. Proc Natl Acad Sci U S A. 1994. PMID: 8278402 Free PMC article. - p53 C-terminal interaction with DNA ends and gaps has opposing effect on specific DNA binding by the core.
Zotchev SB, Protopopova M, Selivanova G. Zotchev SB, et al. Nucleic Acids Res. 2000 Oct 15;28(20):4005-12. doi: 10.1093/nar/28.20.4005. Nucleic Acids Res. 2000. PMID: 11024181 Free PMC article. - Proteolytic cleavage of p53: a model for the activation of p53 in response to DNA damage.
Okorokov AL, Milner J. Okorokov AL, et al. Oncol Res. 1997;9(6-7):267-73. Oncol Res. 1997. PMID: 9406231 Review.
Cited by
- DNA Double-Strand Breaks as Pathogenic Lesions in Neurological Disorders.
Provasek VE, Mitra J, Malojirao VH, Hegde ML. Provasek VE, et al. Int J Mol Sci. 2022 Apr 22;23(9):4653. doi: 10.3390/ijms23094653. Int J Mol Sci. 2022. PMID: 35563044 Free PMC article. Review. - Molecular principles of recruitment and dynamics of guest proteins in liquid droplets.
Kamagata K, Iwaki N, Hazra MK, Kanbayashi S, Banerjee T, Chiba R, Sakomoto S, Gaudon V, Castaing B, Takahashi H, Kimura M, Oikawa H, Takahashi S, Levy Y. Kamagata K, et al. Sci Rep. 2021 Sep 29;11(1):19323. doi: 10.1038/s41598-021-98955-0. Sci Rep. 2021. PMID: 34588591 Free PMC article. - The Rich World of p53 DNA Binding Targets: The Role of DNA Structure.
Brázda V, Fojta M. Brázda V, et al. Int J Mol Sci. 2019 Nov 9;20(22):5605. doi: 10.3390/ijms20225605. Int J Mol Sci. 2019. PMID: 31717504 Free PMC article. Review. - Essential Roles of E3 Ubiquitin Ligases in p53 Regulation.
Sane S, Rezvani K. Sane S, et al. Int J Mol Sci. 2017 Feb 17;18(2):442. doi: 10.3390/ijms18020442. Int J Mol Sci. 2017. PMID: 28218667 Free PMC article. Review. - p53 Specifically Binds Triplex DNA In Vitro and in Cells.
Brázdová M, Tichý V, Helma R, Bažantová P, Polášková A, Krejčí A, Petr M, Navrátilová L, Tichá O, Nejedlý K, Bennink ML, Subramaniam V, Bábková Z, Martínek T, Lexa M, Adámik M. Brázdová M, et al. PLoS One. 2016 Dec 1;11(12):e0167439. doi: 10.1371/journal.pone.0167439. eCollection 2016. PLoS One. 2016. PMID: 27907175 Free PMC article.
References
- Proc Natl Acad Sci U S A. 1975 Jan;72(1):83-7 - PubMed
- Science. 1994 Jul 15;265(5170):346-55 - PubMed
- Oncogene. 1988 Nov;3(5):501-7 - PubMed
- Cell. 1991 Jun 14;65(6):1083-91 - PubMed
- Science. 1991 Jun 21;252(5013):1708-11 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials
Miscellaneous