ATP-dependent transport of glutathione S-conjugates by the multidrug resistance-associated protein - PubMed (original) (raw)

. 1994 Sep 15;54(18):4833-6.

Affiliations

• PMID: 7915193

ATP-dependent transport of glutathione S-conjugates by the multidrug resistance-associated protein

G Jedlitschky et al. Cancer Res. 1994.

Abstract

The ATP-dependent transport of the endogenous glutathione conjugate leukotriene C4 (LTC4) was more than 25-fold higher in membrane vesicles prepared from human leukemia cells (HL60/ADR) overexpressing the multidrug resistance-associated protein than from drug-sensitive parental HL60 cells or revertant cells. Similar results were obtained with S-(2,4-dinitrophenyl)glutathione as substrate. Photoaffinity labeling detected preferentially in the HL60/ADR membranes a 190-kilodalton protein binding [3H]LTC4 and 8-azido[alpha-32P]ATP. The [3H]LTC4-labeled 190-kilodalton protein was immunoprecipitated by an antiserum against the COOH-terminal sequence of multidrug resistance-associated protein. Our results indicate that multidrug resistance-associated protein mediates the ATP-dependent transport of LTC4 and structurally related anionic amphiphilic conjugates.

PubMed Disclaimer

Similar articles

• The MRP gene associated with a non-P-glycoprotein multidrug resistance encodes a 190-kDa membrane bound glycoprotein.
  Krishnamachary N, Center MS. Krishnamachary N, et al. Cancer Res. 1993 Aug 15;53(16):3658-61. Cancer Res. 1993. PMID: 8101765
• Characterization of the ATP-dependent leukotriene C4 export carrier in mastocytoma cells.
  Leier I, Jedlitschky G, Buchholz U, Keppler D. Leier I, et al. Eur J Biochem. 1994 Mar 1;220(2):599-606. doi: 10.1111/j.1432-1033.1994.tb18661.x. Eur J Biochem. 1994. PMID: 8125120
• Transport of leukotriene C4 and structurally related conjugates.
  Jedlitschky G, Keppler D. Jedlitschky G, et al. Vitam Horm. 2002;64:153-84. doi: 10.1016/s0083-6729(02)64005-1. Vitam Horm. 2002. PMID: 11898391 Review.
• [Multidrug resistance].
  Baccarani M. Baccarani M. Haematologica. 1991 Jun;76 Suppl 3:145-9. Haematologica. 1991. PMID: 1684337 Review. Italian. No abstract available.

Cited by

• Ovarian cancer stem cells: a new target for cancer therapy.
  Zhan Q, Wang C, Ngai S. Zhan Q, et al. Biomed Res Int. 2013;2013:916819. doi: 10.1155/2013/916819. Epub 2013 Jan 30. Biomed Res Int. 2013. PMID: 23509802 Free PMC article. Review.
• Development of multidrug-resistance convertors: sense or nonsense?
  van Zuylen L, Nooter K, Sparreboom A, Verweij J. van Zuylen L, et al. Invest New Drugs. 2000 Aug;18(3):205-20. doi: 10.1023/a:1006487003814. Invest New Drugs. 2000. PMID: 10958589 Review.
• The human multidrug resistance protein MRP4 functions as a prostaglandin efflux transporter and is inhibited by nonsteroidal antiinflammatory drugs.
  Reid G, Wielinga P, Zelcer N, van der Heijden I, Kuil A, de Haas M, Wijnholds J, Borst P. Reid G, et al. Proc Natl Acad Sci U S A. 2003 Aug 5;100(16):9244-9. doi: 10.1073/pnas.1033060100. Epub 2003 Jun 30. Proc Natl Acad Sci U S A. 2003. PMID: 12835412 Free PMC article.
• Glutathione conjugation of chlorambucil: measurement and modulation by plant polyphenols.
  Zhang K, Wong KP. Zhang K, et al. Biochem J. 1997 Jul 15;325 ( Pt 2)(Pt 2):417-22. doi: 10.1042/bj3250417. Biochem J. 1997. PMID: 9230122 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Other Literature Sources

  • The Lens - Patent Citations Database

• Molecular Biology Databases

  • Pharmacogenomics Knowledge Base

• Research Materials

  • NCI CPTC Antibody Characterization Program

• Miscellaneous

  • NCI CPTAC Assay Portal