E2A proteins are required for proper B cell development and initiation of immunoglobulin gene rearrangements - PubMed (original) (raw)
E2A proteins are required for proper B cell development and initiation of immunoglobulin gene rearrangements
G Bain et al. Cell. 1994.
Abstract
E12 and E47 are two helix-loop-helix transcription factors that arise by alternative splicing of the E2A gene. Both have been implicated in the regulation of immunoglobulin gene expression. We have now generated E2A (-/-) mice by gene targeting. E2A-null mutant mice fail to generate mature B cells. The arrest of B cell development occurs at an early stage, since no immunoglobulin DJ rearrangements can be detected in homozygous mutant mice. While immunoglobulin germline I mu RAG-1, mb-1, CD19, and lambda 5 transcripts are dramatically reduced in fetal livers of E2A (-/-) mice, B29 and mu degrees transcripts are present, but at lower levels. In addition, we show that Pax-5 transcripts are significantly reduced in fetal livers of E2A (-/-) mice. These data suggest a crucial role for E2A products as central regulators in early B cell differentiation.
Comment in
- Transcriptional control points during lymphopoiesis.
Dorshkind K. Dorshkind K. Cell. 1994 Dec 2;79(5):751-3. doi: 10.1016/0092-8674(94)90065-5. Cell. 1994. PMID: 8001114 Review. No abstract available.
Similar articles
- Coordinate regulation of B cell differentiation by the transcription factors EBF and E2A.
O'Riordan M, Grosschedl R. O'Riordan M, et al. Immunity. 1999 Jul;11(1):21-31. doi: 10.1016/s1074-7613(00)80078-3. Immunity. 1999. PMID: 10435576 - Both E12 and E47 allow commitment to the B cell lineage.
Bain G, Robanus Maandag EC, te Riele HP, Feeney AJ, Sheehy A, Schlissel M, Shinton SA, Hardy RR, Murre C. Bain G, et al. Immunity. 1997 Feb;6(2):145-54. doi: 10.1016/s1074-7613(00)80421-5. Immunity. 1997. PMID: 9047236 - Induction of early B cell factor (EBF) and multiple B lineage genes by the basic helix-loop-helix transcription factor E12.
Kee BL, Murre C. Kee BL, et al. J Exp Med. 1998 Aug 17;188(4):699-713. doi: 10.1084/jem.188.4.699. J Exp Med. 1998. PMID: 9705952 Free PMC article. - B cell differentiation: role of E2A and Pax5/BSAP transcription factors.
Baker SJ, Reddy EP. Baker SJ, et al. Oncogene. 1995 Aug 3;11(3):413-26. Oncogene. 1995. PMID: 7630627 Review. - Regulation of early lymphocyte development by E2A family proteins.
Greenbaum S, Zhuang Y. Greenbaum S, et al. Semin Immunol. 2002 Dec;14(6):405-14. doi: 10.1016/s1044532302000751. Semin Immunol. 2002. PMID: 12457613 Review.
Cited by
- Transcriptional function of E2A, Ebf1, Pax5, Ikaros and Aiolos analyzed by in vivo acute protein degradation in early B cell development.
Fedl AS, Tagoh H, Gruenbacher S, Sun Q, Schenk RL, Froussios K, Jaritz M, Busslinger M, Schwickert TA. Fedl AS, et al. Nat Immunol. 2024 Sep;25(9):1663-1677. doi: 10.1038/s41590-024-01933-7. Epub 2024 Aug 23. Nat Immunol. 2024. PMID: 39179932 - Exclusive Characteristics of the p.E555K Dominant-Negative Variant in Autosomal Dominant E47 Deficiency.
Utsumi T, Tsumura M, Yashiro M, Kato Z, Noma K, Sakura F, Kagawa R, Mizoguchi Y, Karakawa S, Ohnishi H, Cunningham-Rundles C, Arkwright PD, Kobayashi M, Kanegane H, Bogunovic D, Boisson B, Casanova JL, Asano T, Okada S. Utsumi T, et al. J Clin Immunol. 2024 Jul 29;44(7):167. doi: 10.1007/s10875-024-01758-x. J Clin Immunol. 2024. PMID: 39073655 Free PMC article. - The Function of E2A in B-Cell Development.
Miyazaki M, Miyazaki K. Miyazaki M, et al. Adv Exp Med Biol. 2024;1459:97-113. doi: 10.1007/978-3-031-62731-6_5. Adv Exp Med Biol. 2024. PMID: 39017841 Review. - ERG and c-MYC regulate a critical gene network in BCR::ABL1-driven B cell acute lymphoblastic leukemia.
Behrens K, Brajanovski N, Xu Z, Viney EM, DiRago L, Hediyeh-Zadeh S, Davis MJ, Pearson RB, Sanij E, Alexander WS, Ng AP. Behrens K, et al. Sci Adv. 2024 Mar 8;10(10):eadj8803. doi: 10.1126/sciadv.adj8803. Epub 2024 Mar 8. Sci Adv. 2024. PMID: 38457494 Free PMC article. - A landscape of gene expression regulation for synovium in arthritis.
Jiang F, Hu SY, Tian W, Wang NN, Yang N, Dong SS, Song HM, Zhang DJ, Gao HW, Wang C, Wu H, He CY, Zhu DL, Chen XF, Guo Y, Yang Z, Yang TL. Jiang F, et al. Nat Commun. 2024 Feb 15;15(1):1409. doi: 10.1038/s41467-024-45652-x. Nat Commun. 2024. PMID: 38360850 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Research Materials
Miscellaneous