Gamma-irradiation-induced micronuclei from mouse hepatoma cells accumulate high levels of the tumor suppressor protein p53 - PubMed (original) (raw)
. 1994 Jul 15;54(14):3651-5.
Affiliations
- PMID: 8033078
Gamma-irradiation-induced micronuclei from mouse hepatoma cells accumulate high levels of the tumor suppressor protein p53
C Unger et al. Cancer Res. 1994.
Abstract
The p53 tumor suppressor protein plays an important role in the G1 arrest of cells treated with DNA-damaging agents. Mouse hepatoma cells with wild-type or mutated p53 genotype were gamma-irradiated and the time course of p53 expression was determined by immunocytochemical staining. In p53 wild-type cells, gamma-irradiation led to a transient accumulation of the protein in the nuclei, whereas no such accumulation occurred in p53-mutated cells. Micronuclei were induced by gamma-irradiation in both wild-type and mutated cells in a dose- and time-dependent manner, but only micronuclei from p53 wild-type cells demonstrated a strongly positive staining reaction for p53 protein. This accumulation of p53 protein in micronuclei was not associated with a block in DNA synthesis as evidenced by bromodeoxyuridine labeling experiments.
Similar articles
- Wild-type function of the p53 tumor suppressor protein is not required for apoptosis of mouse hepatoma cells.
Unger C, Buchmann A, Bünemann CL, Kress S, Schwarz M. Unger C, et al. Cell Death Differ. 1998 Jan;5(1):87-95. doi: 10.1038/sj.cdd.4400321. Cell Death Differ. 1998. PMID: 10200449 - Role of wild type p53 in the G2 phase: regulation of the gamma-irradiation-induced delay and DNA repair.
Schwartz D, Almog N, Peled A, Goldfinger N, Rotter V. Schwartz D, et al. Oncogene. 1997 Nov 20;15(21):2597-607. doi: 10.1038/sj.onc.1201436. Oncogene. 1997. PMID: 9399647 - Role of p53 in G2/M cell cycle arrest and apoptosis in response to gamma-irradiation in ovarian carcinoma cell lines.
Concin N, Stimpfl M, Zeillinger C, Wolff U, Hefler L, Sedlak J, Leodolter S, Zeillinger R. Concin N, et al. Int J Oncol. 2003 Jan;22(1):51-7. Int J Oncol. 2003. PMID: 12469184 - Intracellular localization of p53 tumor suppressor protein in gamma-irradiated cells is cell cycle regulated and determined by the nucleus.
Komarova EA, Zelnick CR, Chin D, Zeremski M, Gleiberman AS, Bacus SS, Gudkov AV. Komarova EA, et al. Cancer Res. 1997 Dec 1;57(23):5217-20. Cancer Res. 1997. PMID: 9393737 - p53 accumulates in micronuclei after treatment with a DNA breaking chemical, methylnitrosourea, and with the spindle poison, vinblastine.
Granetto C, Ottaggio L, Abbondandolo A, Bonatti S. Granetto C, et al. Mutat Res. 1996 Jun 10;352(1-2):61-4. doi: 10.1016/0027-5107(95)00235-9. Mutat Res. 1996. PMID: 8676917
Cited by
- Genetic instability as a consequence of inappropriate entry into and progression through S-phase.
Almasan A, Linke SP, Paulson TG, Huang LC, Wahl GM. Almasan A, et al. Cancer Metastasis Rev. 1995 Mar;14(1):59-73. doi: 10.1007/BF00690212. Cancer Metastasis Rev. 1995. PMID: 7606822 Review. - Late G1 accumulation after 2 Gy of gamma-irradiation is related to endogenous Raf-1 protein expression and intrinsic radiosensitivity in human cells.
Warenius HM, Jones M, Jones MD, Browning PG, Seabra LA, Thompson CC. Warenius HM, et al. Br J Cancer. 1998 Apr;77(8):1220-8. doi: 10.1038/bjc.1998.206. Br J Cancer. 1998. PMID: 9579826 Free PMC article. - Chronology of p53 protein accumulation in gastric carcinogenesis.
Craanen ME, Blok P, Dekker W, Offerhaus GJ, Tytgat GN. Craanen ME, et al. Gut. 1995 Jun;36(6):848-52. doi: 10.1136/gut.36.6.848. Gut. 1995. PMID: 7615272 Free PMC article. - Deficiency of retinoblastoma protein leads to inappropriate S-phase entry, activation of E2F-responsive genes, and apoptosis.
Almasan A, Yin Y, Kelly RE, Lee EY, Bradley A, Li W, Bertino JR, Wahl GM. Almasan A, et al. Proc Natl Acad Sci U S A. 1995 Jun 6;92(12):5436-40. doi: 10.1073/pnas.92.12.5436. Proc Natl Acad Sci U S A. 1995. PMID: 7777526 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Research Materials
Miscellaneous