Interspersion of repetitive and nonrepetitive DNA sequences in the Drosophila melanogaster genome - PubMed (original) (raw)
Interspersion of repetitive and nonrepetitive DNA sequences in the Drosophila melanogaster genome
J E Manning et al. Cell. 1975 Feb.
Abstract
Cot analysis shows that the haploid Drosophila genome contains 12 percent rapidly reassociating, highly reiterated DNA, 12 percent middle repetitive DNA with an average reiteration frequency of 70, and 70 percent single-copy DNA. The distribution of the middle repetitive sequences in the genome has been studied by an examination in the electron microscope of the structures obtained when middle repetitive sequences present on large DNA strands reassociate and by the hydroxyapatite binding methods developed by Davidson et al. (1973). At least one third by weight of the middle repetitive sequences are interspersed in single-copy sequences. These interspersed middle repetitive sequences have a fairly uniform distribution of lengths from less than 0.5 to 13 kb, with a number average value of 5.6 kb. The average distance between middle repetitive sequences is greater than 13 kb. The data do not exclude the possibility that essentially all of the middle repetitive sequences have the interspersion pattern described above; however, it is possible that some of the middle repetitive sequences of Drosophila are clustered in stretches of length much greater than 13 kb. The interspersion pattern of the middle repetitive sequences in Drosophila is quite different from that which occurs in the sea urchin, in Xenopus, in rat, and probably many other higher eucaryotes.
Similar articles
- Sequence organisation in nuclear DNA from Physarum polycephalum. Interspersion of repetitive and single-copy sequences.
Hardman N, Jack PL, Fergie RC, Gerrie LM. Hardman N, et al. Eur J Biochem. 1980 Jan;103(2):247-57. doi: 10.1111/j.1432-1033.1980.tb04309.x. Eur J Biochem. 1980. PMID: 7363891 - Organization of repetitive DNA sequences in the genome of the echinoderm Holothuria tubulosa.
Sainz J, Prats E, Ruiz S, Cornudella L. Sainz J, et al. Biochimie. 1992 Dec;74(12):1067-74. doi: 10.1016/0300-9084(92)90004-x. Biochimie. 1992. PMID: 1292614 - Structural genes adjacent to interspersed repetitive DNA sequences.
Davidson EH, Hough BR, Klein WH, Britten RJ. Davidson EH, et al. Cell. 1975 Mar;4(3):217-38. doi: 10.1016/0092-8674(75)90170-1. Cell. 1975. PMID: 1122554 - DNA of Drosophila chromosomes.
Laird CD. Laird CD. Annu Rev Genet. 1973;7:177-204. doi: 10.1146/annurev.ge.07.120173.001141. Annu Rev Genet. 1973. PMID: 4593302 Review. No abstract available. - DNA sequence arrangement and preliminary evidence on its evolution.
Britten RJ, Davidson EH. Britten RJ, et al. Fed Proc. 1976 Aug;35(10):2151-7. Fed Proc. 1976. PMID: 780132 Review.
Cited by
- Locations of chromosomal proteins in polytene chromosomes.
Alfageme CR, Rudkin GT, Cohen LH. Alfageme CR, et al. Proc Natl Acad Sci U S A. 1976 Jun;73(6):2038-42. doi: 10.1073/pnas.73.6.2038. Proc Natl Acad Sci U S A. 1976. PMID: 819929 Free PMC article. - Nature of the heterogeneity in mispairing of reannealed middle-repetitive fern DNA.
Bouchard RA, Swift H. Bouchard RA, et al. Chromosoma. 1977 Jun 23;61(4):317-33. doi: 10.1007/BF00288616. Chromosoma. 1977. PMID: 880840 No abstract available. - Interspecific "common" repetitive DNA sequences in salamanders of the genus Plethodon.
Mizuno S, Andrews C, Macgregor HC. Mizuno S, et al. Chromosoma. 1976 Oct 12;58(1):1-31. doi: 10.1007/BF00293437. Chromosoma. 1976. PMID: 1001148 - Gene mapping and gene enrichment by the avidin-biotin interaction: use of cytochrome-c as a polyamine bridge.
Sodja A, Davidson N. Sodja A, et al. Nucleic Acids Res. 1978 Feb;5(2):385-401. doi: 10.1093/nar/5.2.385. Nucleic Acids Res. 1978. PMID: 204910 Free PMC article. - Analysis of two cosmid clones from chromosome 4 of Drosophila melanogaster reveals two new genes amid an unusual arrangement of repeated sequences.
Locke J, Podemski L, Roy K, Pilgrim D, Hodgetts R. Locke J, et al. Genome Res. 1999 Feb;9(2):137-49. Genome Res. 1999. PMID: 10022978 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases
Miscellaneous