Blockage of chemotactic peptide-induced stimulation of neutrophils by wortmannin as a result of selective inhibition of phosphatidylinositol 3-kinase - PubMed (original) (raw)
. 1994 Feb 4;269(5):3563-7.
Affiliations
- PMID: 8106399
Free article
Blockage of chemotactic peptide-induced stimulation of neutrophils by wortmannin as a result of selective inhibition of phosphatidylinositol 3-kinase
T Okada et al. J Biol Chem. 1994.
Free article
Abstract
Wortmannin, a fungal metabolite, inhibited 32P labeling of phosphatidylinositol trisphosphate, a product of phosphatidylinositol 3-kinase (PI 3-kinase), selectively in formyl peptide-stimulated 32P-loaded guinea pig neutrophils. The inhibition was of the same concentration dependence (with the half-maximal inhibition around 50 nM) as was observed for the simultaneous inhibition of formyl peptide-induced superoxide anion production. Wortmannin inhibited all three of the PI 3-kinase activities found in the cytosol fraction of guinea pig neutrophils, with a similar dose dependence (the half-maximal effects at 5 nM). Wortmannin was also effective on an immunologically purified preparation of the enzyme. The inhibition was of a noncompetitive type with regard to ATP and was observed consistently when PI, PI monophosphate, or PI bisphosphate was used as substrate. PI 4-kinase activity was not affected. It is concluded, therefore, that wortmannin abolished the formyl peptide-induced stimulation of neutrophils as a result of the inhibition of PI 3-kinase. An essential role of PI 3-kinase in receptor-mediated signaling in neutrophils thus evidenced with the use of wortmannin will be expanded to other cellular signaling systems.
Similar articles
- Wortmannin is a potent phosphatidylinositol 3-kinase inhibitor: the role of phosphatidylinositol 3,4,5-trisphosphate in neutrophil responses.
Arcaro A, Wymann MP. Arcaro A, et al. Biochem J. 1993 Dec 1;296 ( Pt 2)(Pt 2):297-301. doi: 10.1042/bj2960297. Biochem J. 1993. PMID: 8257416 Free PMC article. - Wortmannin as a unique probe for an intracellular signalling protein, phosphoinositide 3-kinase.
Ui M, Okada T, Hazeki K, Hazeki O. Ui M, et al. Trends Biochem Sci. 1995 Aug;20(8):303-7. doi: 10.1016/s0968-0004(00)89056-8. Trends Biochem Sci. 1995. PMID: 7667888 Review. - Lipids on the move: phosphoinositide 3-kinases in leukocyte function.
Wymann MP, Sozzani S, Altruda F, Mantovani A, Hirsch E. Wymann MP, et al. Immunol Today. 2000 Jun;21(6):260-4. doi: 10.1016/s0167-5699(00)01649-2. Immunol Today. 2000. PMID: 10939787 Review. No abstract available.
Cited by
- Affinity enhancement of polo-like kinase 1 polo box domain-binding ligands by a bivalent approach using a covalent kinase-binding component.
Tsuji K, Tamamura H, Burke TR Jr. Tsuji K, et al. RSC Chem Biol. 2024 Jun 4;5(8):721-728. doi: 10.1039/d4cb00031e. eCollection 2024 Jul 31. RSC Chem Biol. 2024. PMID: 39092437 Free PMC article. - Signaling Pathways in Cancer: Therapeutic Targets, Combinatorial Treatments, and New Developments.
Yip HYK, Papa A. Yip HYK, et al. Cells. 2021 Mar 16;10(3):659. doi: 10.3390/cells10030659. Cells. 2021. PMID: 33809714 Free PMC article. Review. - cPLA2α-/- sympathetic neurons exhibit increased membrane excitability and loss of N-Type Ca2+ current inhibition by M1 muscarinic receptor signaling.
Liu L, Bonventre JV, Rittenhouse AR. Liu L, et al. PLoS One. 2018 Dec 17;13(12):e0201322. doi: 10.1371/journal.pone.0201322. eCollection 2018. PLoS One. 2018. PMID: 30557348 Free PMC article. - Human mesenchymal stromal cells in adhesion to cell-derived extracellular matrix and titanium: Comparative kinome profile analysis.
Baroncelli M, Fuhler GM, van de Peppel J, Zambuzzi WF, van Leeuwen JP, van der Eerden BCJ, Peppelenbosch MP. Baroncelli M, et al. J Cell Physiol. 2019 Mar;234(3):2984-2996. doi: 10.1002/jcp.27116. Epub 2018 Jul 30. J Cell Physiol. 2019. PMID: 30058720 Free PMC article. - Monophosphoryl lipid A prevents impairment of medullary thick ascending limb [Formula: see text] absorption and improves plasma [Formula: see text] concentration in septic mice.
Watts BA, George T, Sherwood ER, Good DW. Watts BA, et al. Am J Physiol Renal Physiol. 2018 Sep 1;315(3):F711-F725. doi: 10.1152/ajprenal.00033.2018. Epub 2018 May 9. Am J Physiol Renal Physiol. 2018. PMID: 29741098 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Research Materials
Miscellaneous