A translation-attenuating intraleader open reading frame is selected on coronavirus mRNAs during persistent infection - PubMed (original) (raw)

A translation-attenuating intraleader open reading frame is selected on coronavirus mRNAs during persistent infection

M A Hofmann et al. Proc Natl Acad Sci U S A. 1993.

Abstract

Short open reading frames within the 5' leader of some eukaryotic mRNAs are known to regulate the rate of translation initiation on the downstream open reading frame. By employing the polymerase chain reaction, we learned that the 5'-terminal 5 nt on the common leader sequence of bovine coronavirus subgenomic mRNAs were heterogeneous and hypervariable throughout early infection in cell culture and that as a persistent infection became established, termini giving rise to a common 33-nt intraleader open reading frame were selected. Since the common leader is derived from the genomic 5' end during transcription, a common focus of origin for the heterogeneity is expected. The intraleader open reading frame was shown by in vitro translation studies to attenuate translation of downstream open reading frames in a cloned bovine coronavirus mRNA molecule. Selection of an intraleader open reading frame resulting in a general attenuation of mRNA translation and a consequent attenuation of virus replication may, therefore, be a mechanism by which coronaviruses and possibly other RNA viruses with a similar transcriptional strategy maintain a persistent infection.

PubMed Disclaimer

Similar articles

Cited by

References

    1. Curr Top Microbiol Immunol. 1981;93:125-49 - PubMed
    1. Proc Natl Acad Sci U S A. 1978 Oct;75(10):4886-90 - PubMed
    1. Nucleic Acids Res. 1983 Sep 24;11(18):6409-18 - PubMed
    1. EMBO J. 1983;2(10):1839-44 - PubMed
    1. Proc Natl Acad Sci U S A. 1984 Jun;81(12):3626-30 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources