Thick-section fluorescence in situ hybridization on formalin-fixed, paraffin-embedded archival tissue provides a histogenetic profile - PubMed (original) (raw)

. 1994 Feb;144(2):237-43.

Affiliations

Thick-section fluorescence in situ hybridization on formalin-fixed, paraffin-embedded archival tissue provides a histogenetic profile

C T Thompson et al. Am J Pathol. 1994 Feb.

Abstract

Fluorescence in situ hybridization has become a major tool for analysis of gene and chromosome copy number in normal and malignant tissue. The technique has been applied widely to fresh tissue and dispersed formalin-fixed, paraffin-embedded archival tissue, but its use on sections of archival tissue has largely been limited to sections < 6 mu thick. This does not provide intact, uncut nuclei for accurate analysis of gene or chromosome copy number. We report here a method of hybridization to sections > 20 microns thick that overcomes these difficulties. Key developments were the use of DNA probes directly labeled with fluorochromes and optical sectioning using laser-scanning confocal microscopy.

PubMed Disclaimer

References

    1. Cancer Chemother Rep. 1966 Mar;50(3):125-8 - PubMed
    1. Am J Clin Pathol. 1993 Jun;99(6):714-20 - PubMed
    1. J Immunol Methods. 1981;43(3):349-50 - PubMed
    1. Proc Natl Acad Sci U S A. 1986 May;83(9):2934-8 - PubMed
    1. Exp Cell Res. 1988 Jun;176(2):199-220 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources