Expression, identification and subcellular localization of the proteins encoded by the hepatitis C viral genome - PubMed (original) (raw)

Expression, identification and subcellular localization of the proteins encoded by the hepatitis C viral genome

M J Selby et al. J Gen Virol. 1993 Jun.

Abstract

We have expressed the full-length coding region and selected domains of the hepatitis C virus (HCV) cDNA in mammalian cells by transfection. Using HCV antibody-positive human sera and monospecific antibodies the proteins encoded by the putative structural and non-structural regions of the open reading frame of HCV were identified as core (p22), E1 (gp32-35), E2 (gp68-72), NS2 (p23), NS3 (p72), NS4a and b (p10 and p27) and NS5a and b (p56 and p70). We have also defined the subcellular localizations of the HCV proteins using indirect immunofluorescence assays.

PubMed Disclaimer

Similar articles

• Expression and identification of hepatitis C virus polyprotein cleavage products.
  Grakoui A, Wychowski C, Lin C, Feinstone SM, Rice CM. Grakoui A, et al. J Virol. 1993 Mar;67(3):1385-95. doi: 10.1128/JVI.67.3.1385-1395.1993. J Virol. 1993. PMID: 7679746 Free PMC article.
• Expression and characterization of glycoprotein gp35 of hepatitis C virus using recombinant vaccinia virus.
  Kohara M, Tsukiyama-Kohara K, Maki N, Asano K, Yamaguchi K, Miki K, Tanaka S, Hattori N, Matsuura Y, Saito I, et al. Kohara M, et al. J Gen Virol. 1992 Sep;73 ( Pt 9):2313-8. doi: 10.1099/0022-1317-73-9-2313. J Gen Virol. 1992. PMID: 1328487
• Production of nonstructural proteins of hepatitis C virus requires a putative viral protease encoded by NS3.
  Manabe S, Fuke I, Tanishita O, Kaji C, Gomi Y, Yoshida S, Mori C, Takamizawa A, Yosida I, Okayama H. Manabe S, et al. Virology. 1994 Feb;198(2):636-44. doi: 10.1006/viro.1994.1075. Virology. 1994. PMID: 8291245
• [Virology of hepatitis C virus].
  Hofmann H. Hofmann H. Wien Med Wochenschr. 2000;150(23-24):463-6. Wien Med Wochenschr. 2000. PMID: 11205175 Review. German.
• Processing pathways of the hepatitis C virus proteins.
  Lohmann V, Koch JO, Bartenschlager R. Lohmann V, et al. J Hepatol. 1996;24(2 Suppl):11-9. J Hepatol. 1996. PMID: 8836884 Review.

Cited by

• Hepatitis C virus core protein decreases lipid droplet turnover: a mechanism for core-induced steatosis.
  Harris C, Herker E, Farese RV Jr, Ott M. Harris C, et al. J Biol Chem. 2011 Dec 9;286(49):42615-42625. doi: 10.1074/jbc.M111.285148. Epub 2011 Oct 7. J Biol Chem. 2011. PMID: 21984835 Free PMC article.
• Hepatitis C virus NS3 protein can activate the Notch-signaling pathway through binding to a transcription factor, SRCAP.
  Iwai A, Takegami T, Shiozaki T, Miyazaki T. Iwai A, et al. PLoS One. 2011;6(6):e20718. doi: 10.1371/journal.pone.0020718. Epub 2011 Jun 6. PLoS One. 2011. PMID: 21673954 Free PMC article.
• Antiviral activity of Acacia nilotica against Hepatitis C Virus in liver infected cells.
  Rehman S, Ashfaq UA, Riaz S, Javed T, Riazuddin S. Rehman S, et al. Virol J. 2011 May 12;8:220. doi: 10.1186/1743-422X-8-220. Virol J. 2011. PMID: 21569385 Free PMC article.
• Efficient hepatitis C virus particle formation requires diacylglycerol acyltransferase-1.
  Herker E, Harris C, Hernandez C, Carpentier A, Kaehlcke K, Rosenberg AR, Farese RV Jr, Ott M. Herker E, et al. Nat Med. 2010 Nov;16(11):1295-8. doi: 10.1038/nm.2238. Epub 2010 Oct 10. Nat Med. 2010. PMID: 20935628 Free PMC article.
• The Hepatitis C Virus NS5A Stimulates NS5B During In Vitro RNA Synthesis in a Template Specific Manner.
  Quezada EM, Kane CM. Quezada EM, et al. Open Biochem J. 2009 Apr 20;3:39-48. doi: 10.2174/1874091X00903010039. Open Biochem J. 2009. PMID: 19590581 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Ingenta plc

• Other Literature Sources

  • The Lens - Patent Citations Database

• Research Materials

  • NCI CPTC Antibody Characterization Program