Surface phenotype and functions of tumor-infiltrating dendritic cells: CD8 expression by a cell subpopulation - PubMed (original) (raw)
. 1993 Oct;23(10):2517-25.
doi: 10.1002/eji.1830231021.
Affiliations
- PMID: 8405052
- DOI: 10.1002/eji.1830231021
Surface phenotype and functions of tumor-infiltrating dendritic cells: CD8 expression by a cell subpopulation
P Chaux et al. Eur J Immunol. 1993 Oct.
Abstract
Although the function and significance of tumor-infiltrating dendritic cells (TIDC) in the immune response to tumor have never been clearly demonstrated, their location suggests that they play a critical role in the presentation of tumor antigen to specific T cells. We studied the morphological and functional characteristics of interstitial dendritic cells (DC) located inside tumors obtained by injection of cancer cells into syngeneic rats. Single and double immunostaining of tumor sections revealed a dense network of cells which expressed class II major histocompatibility complex (MHC II) molecules. Cell morphology and surface markers were characteristic of DC populations in other tissues. These DC were in close contact with tumor cells and increased in number as the tumor grew larger. Unexpectedly, a subpopulation of morphologically characteristic TIDC expressed both CD8 and MHC II molecules. TIDC were purified from tumors by gradient centrifugation and immunobeads and characterized by morphology, ultrastructural study and surface markers studied by flow cytometry. TIDC were negative for the CD5 molecule (a pan T cell marker), and were not labeled with 3.2.3 monoclonal antibody (mAb) (an NK cell marker) or with Ki-M2R mAb (a macrophage marker). A subpopulation of TIDC expressed the CD8 molecule, confirming the in situ results. TIDC expressed high levels of class I and class II MHC molecules and the adhesion molecule ICAM-1. This expression is compatible with effective antigen presenting function. Purified TIDC triggered rapid and high levels of proliferation of tumor-immune T cells in vitro, demonstrating the potential of these cells to constitutively process and present tumor-associated antigens.
Similar articles
- Human hepatoma cells expressing MHC antigens display accessory cell function: dependence on LFA-1/ICAM-1 interaction.
Paroli M, Carloni G, Franco A, De Petrillo G, Alfani E, Perrone A, Barnaba V. Paroli M, et al. Immunology. 1994 Jun;82(2):215-21. Immunology. 1994. PMID: 7927492 Free PMC article. - Immune selection in murine tumors. Ph.d thesis.
Svane IM, Engel AM. Svane IM, et al. APMIS Suppl. 2003;(106):1-46. APMIS Suppl. 2003. PMID: 12739251 - Bacterial antigen delivery systems: phagocytic processing of bacterial antigens for MHC-I and MHC-II presentation to T cells.
Svensson M, Pfeifer J, Stockinger B, Wick MJ. Svensson M, et al. Behring Inst Mitt. 1997 Feb;(98):197-211. Behring Inst Mitt. 1997. PMID: 9382741 Review. - Escape from host-antitumor immunity.
Pawelec G, Zeuthen J, Kiessling R. Pawelec G, et al. Crit Rev Oncog. 1997;8(2-3):111-41. doi: 10.1615/critrevoncog.v8.i2-3.10. Crit Rev Oncog. 1997. PMID: 9570292 Review.
Cited by
- Immunomodulatory effects of cyclophosphamide and implementations for vaccine design.
Sistigu A, Viaud S, Chaput N, Bracci L, Proietti E, Zitvogel L. Sistigu A, et al. Semin Immunopathol. 2011 Jul;33(4):369-83. doi: 10.1007/s00281-011-0245-0. Epub 2011 May 25. Semin Immunopathol. 2011. PMID: 21611872 Review. - CD4+CD25+ Tregs control the TRAIL-dependent cytotoxicity of tumor-infiltrating DCs in rodent models of colon cancer.
Roux S, Apetoh L, Chalmin F, Ladoire S, Mignot G, Puig PE, Lauvau G, Zitvogel L, Martin F, Chauffert B, Yagita H, Solary E, Ghiringhelli F. Roux S, et al. J Clin Invest. 2008 Nov;118(11):3751-61. doi: 10.1172/JCI35890. Epub 2008 Oct 1. J Clin Invest. 2008. PMID: 18830416 Free PMC article. - Immunohistochemical localization of CD1a-positive putative dendritic cells in human breast tumours.
Hillenbrand EE, Neville AM, Coventry BJ. Hillenbrand EE, et al. Br J Cancer. 1999 Feb;79(5-6):940-4. doi: 10.1038/sj.bjc.6690150. Br J Cancer. 1999. PMID: 10070894 Free PMC article. - Expression of fibronectin ED-A+ and ED-B+ isoforms by human and experimental colorectal cancer. Contribution of cancer cells and tumor-associated myofibroblasts.
Pujuguet P, Hammann A, Moutet M, Samuel JL, Martin F, Martin M. Pujuguet P, et al. Am J Pathol. 1996 Feb;148(2):579-92. Am J Pathol. 1996. PMID: 8579120 Free PMC article. - Immune response of the skin.
Schmitt D. Schmitt D. Clin Rev Allergy Immunol. 1995 Fall;13(3):177-88. doi: 10.1007/BF02771759. Clin Rev Allergy Immunol. 1995. PMID: 8535925 Review. No abstract available.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Research Materials
Miscellaneous