Regulation of the human hsp70 promoter by p53 - PubMed (original) (raw)

Regulation of the human hsp70 promoter by p53

S N Agoff et al. Science. 1993.

Abstract

The tumor suppressor p53 is a nuclear phosphoprotein with characteristics of a transcription factor. It displays sequence-specific DNA binding, contains a potent transactivation domain, and has been implicated as both a transcriptional activator and a repressor. Transcription of the human hsp70 gene is stimulated by adenovirus E1a protein. This E1a transactivation of the hsp70 promoter is mediated by CCAAT binding factor (CBF). It is demonstrated here that p53 both represses transcription from the human hsp70 promoter and also interacts with CBF. Thus, the repression of the hsp70 promoter by p53 may be mediated by direct protein-protein interaction with CBF. These results suggest that protein-protein interaction between p53 and specific transcription factors may be an additional mechanism by which p53 regulates gene expression.

PubMed Disclaimer

Similar articles

• Two domains of p53 interact with the TATA-binding protein, and the adenovirus 13S E1A protein disrupts the association, relieving p53-mediated transcriptional repression.
  Horikoshi N, Usheva A, Chen J, Levine AJ, Weinmann R, Shenk T. Horikoshi N, et al. Mol Cell Biol. 1995 Jan;15(1):227-34. doi: 10.1128/MCB.15.1.227. Mol Cell Biol. 1995. PMID: 7799929 Free PMC article.
• Transcriptional repression by p53 involves molecular interactions distinct from those with the TATA box binding protein.
  Farmer G, Friedlander P, Colgan J, Manley JL, Prives C. Farmer G, et al. Nucleic Acids Res. 1996 Nov 1;24(21):4281-8. doi: 10.1093/nar/24.21.4281. Nucleic Acids Res. 1996. PMID: 8932384 Free PMC article.
• Cooperative DNA binding of p53 with TFIID (TBP): a possible mechanism for transcriptional activation.
  Chen X, Farmer G, Zhu H, Prywes R, Prives C. Chen X, et al. Genes Dev. 1993 Oct;7(10):1837-49. doi: 10.1101/gad.7.10.1837. Genes Dev. 1993. PMID: 8405994
• Interaction of the Dr1 inhibitory factor with the TATA binding protein is disrupted by adenovirus E1A.
  Kraus VB, Inostroza JA, Yeung K, Reinberg D, Nevins JR. Kraus VB, et al. Proc Natl Acad Sci U S A. 1994 Jul 5;91(14):6279-82. doi: 10.1073/pnas.91.14.6279. Proc Natl Acad Sci U S A. 1994. PMID: 8022773 Free PMC article.
• Transcriptional regulation of the p53 tumor suppressor gene: a potential target for cancer therapeutics?
  Reisman D, Boggs K. Reisman D, et al. Recent Pat DNA Gene Seq. 2007;1(3):176-85. doi: 10.2174/187221507782360236. Recent Pat DNA Gene Seq. 2007. PMID: 19075931 Review.

Cited by

• The tumor suppressor p53 inhibits Net, an effector of Ras/extracellular signal-regulated kinase signaling.
  Nakade K, Zheng H, Ganguli G, Buchwalter G, Gross C, Wasylyk B. Nakade K, et al. Mol Cell Biol. 2004 Feb;24(3):1132-42. doi: 10.1128/MCB.24.3.1132-1142.2004. Mol Cell Biol. 2004. PMID: 14729959 Free PMC article.
• HIV-1-infected astrocytes and the microglial proteome.
  Wang T, Gong N, Liu J, Kadiu I, Kraft-Terry SD, Schlautman JD, Ciborowski P, Volsky DJ, Gendelman HE. Wang T, et al. J Neuroimmune Pharmacol. 2008 Sep;3(3):173-86. doi: 10.1007/s11481-008-9110-x. Epub 2008 Jun 28. J Neuroimmune Pharmacol. 2008. PMID: 18587649 Free PMC article.
• Sp1-mediated transcription of the Werner helicase gene is modulated by Rb and p53.
  Yamabe Y, Shimamoto A, Goto M, Yokota J, Sugawara M, Furuichi Y. Yamabe Y, et al. Mol Cell Biol. 1998 Nov;18(11):6191-200. doi: 10.1128/MCB.18.11.6191. Mol Cell Biol. 1998. PMID: 9774636 Free PMC article.
• Transcriptional activation of the human epidermal growth factor receptor promoter by human p53.
  Ludes-Meyers JH, Subler MA, Shivakumar CV, Munoz RM, Jiang P, Bigger JE, Brown DR, Deb SP, Deb S. Ludes-Meyers JH, et al. Mol Cell Biol. 1996 Nov;16(11):6009-19. doi: 10.1128/MCB.16.11.6009. Mol Cell Biol. 1996. PMID: 8887630 Free PMC article.
• Transcriptional regulation of basic fibroblast growth factor gene by p53 in human glioblastoma and hepatocellular carcinoma cells.
  Ueba T, Nosaka T, Takahashi JA, Shibata F, Florkiewicz RZ, Vogelstein B, Oda Y, Kikuchi H, Hatanaka M. Ueba T, et al. Proc Natl Acad Sci U S A. 1994 Sep 13;91(19):9009-13. doi: 10.1073/pnas.91.19.9009. Proc Natl Acad Sci U S A. 1994. PMID: 8090761 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Atypon
  • Ovid Technologies, Inc.

• Other Literature Sources

  • The Lens - Patent Citations Database

• Research Materials

  • NCI CPTC Antibody Characterization Program

• Miscellaneous

  • NCI CPTAC Assay Portal
  • SciCrunch