Predisposition to neoplastic transformation caused by gene replacement of H-ras1 - PubMed (original) (raw)

. 1993 Jun 4;260(5113):1524-7.

doi: 10.1126/science.8502998.

Affiliations

• PMID: 8502998
• DOI: 10.1126/science.8502998

Predisposition to neoplastic transformation caused by gene replacement of H-ras1

R E Finney et al. Science. 1993.

Abstract

Homologous recombination was used to introduce a nominally transforming mutation into an endogenous H-ras1 gene in Rat1 fibroblasts. Although both the mutant and the remaining normal allele were expressed equally, the heterozygous cells were not neoplastically transformed. Instead, spontaneously transformed cells arose from the heterozygotes at a low frequency, and the majority of these cells had amplified the mutant allele. Thus, the activated H-ras1 allele was not by itself dominant over the normal allele but predisposed cells to transformation by independent events, such as amplification of the mutant allele.

PubMed Disclaimer

Publication types

MeSH terms

LinkOut - more resources

• Full Text Sources

  • Atypon
  • Ovid Technologies, Inc.

• Other Literature Sources

  • The Lens - Patent Citations Database

• Research Materials

  • Cellosaurus - a cell line knowledge resource
  • NCI CPTC Antibody Characterization Program

• Miscellaneous

  • NCI CPTAC Assay Portal