The transactivator proteins VP16 and GAL4 bind replication factor A - PubMed (original) (raw)
The transactivator proteins VP16 and GAL4 bind replication factor A
Z He et al. Cell. 1993.
Abstract
Many transcription factors can activate the initiation of DNA replication. We have used affinity chromatography to show that the acidic activation domains of the transcription factors VP16, GAL4, and p53 each bind selectively to human and yeast replication factor A (RPA). The binding is direct and to the largest subunit of the trimeric RPA complex, RPA-1. Mutations in VP16 that reduce the ability of GAL4-VP16 to activate polyomavirus DNA replication also compromise the binding of VP16 to RPA. We suggest that transcription factors may interact with RPA either to stabilize single-stranded DNA at a replication origin or to recruit DNA polymerase alpha to the replication initiation complex.
Similar articles
- Transcriptional activation by DNA-binding derivatives of HSV-1 VP16 that lack the carboxyl-terminal acidic activation domain.
Popova B, Bilan P, Xiao P, Faught M, Capone JP. Popova B, et al. Virology. 1995 May 10;209(1):19-28. doi: 10.1006/viro.1995.1227. Virology. 1995. PMID: 7747469 - Characterization of the interaction between the acidic activation domain of VP16 and the RNA polymerase II initiation factor TFIIB.
Gupta R, Emili A, Pan G, Xiao H, Shales M, Greenblatt J, Ingles CJ. Gupta R, et al. Nucleic Acids Res. 1996 Jun 15;24(12):2324-30. doi: 10.1093/nar/24.12.2324. Nucleic Acids Res. 1996. PMID: 8710503 Free PMC article. - Activation of chromosomal DNA replication in Saccharomyces cerevisiae by acidic transcriptional activation domains.
Li R, Yu DS, Tanaka M, Zheng L, Berger SL, Stillman B. Li R, et al. Mol Cell Biol. 1998 Mar;18(3):1296-302. doi: 10.1128/MCB.18.3.1296. Mol Cell Biol. 1998. PMID: 9488444 Free PMC article. - The transactivator GAL4: co-activators, adaptors and chromatin.
Stanway CA. Stanway CA. Bioessays. 1991 May;13(5):241-2. doi: 10.1002/bies.950130507. Bioessays. 1991. PMID: 1892477 Review. No abstract available. - Cooperative assembly of RNA polymerase II transcription complexes.
Ellwood K, Chi T, Huang W, Mitsouras K, Carey M. Ellwood K, et al. Cold Spring Harb Symp Quant Biol. 1998;63:253-61. doi: 10.1101/sqb.1998.63.253. Cold Spring Harb Symp Quant Biol. 1998. PMID: 10384289 Review. No abstract available.
Cited by
- Saccharomyces cerevisiae replication protein A binds to single-stranded DNA in multiple salt-dependent modes.
Kumaran S, Kozlov AG, Lohman TM. Kumaran S, et al. Biochemistry. 2006 Oct 3;45(39):11958-73. doi: 10.1021/bi060994r. Biochemistry. 2006. PMID: 17002295 Free PMC article. - DNA replication but not nucleotide excision repair is required for UVC-induced replication protein A phosphorylation in mammalian cells.
Rodrigo G, Roumagnac S, Wold MS, Salles B, Calsou P. Rodrigo G, et al. Mol Cell Biol. 2000 Apr;20(8):2696-705. doi: 10.1128/MCB.20.8.2696-2705.2000. Mol Cell Biol. 2000. PMID: 10733572 Free PMC article. - The role of the carboxyterminal domain of RNA polymerase II in regulating origins of DNA replication in Saccharomyces cerevisiae.
Gauthier L, Dziak R, Kramer DJ, Leishman D, Song X, Ho J, Radovic M, Bentley D, Yankulov K. Gauthier L, et al. Genetics. 2002 Nov;162(3):1117-29. doi: 10.1093/genetics/162.3.1117. Genetics. 2002. PMID: 12454060 Free PMC article. - Bovine papillomavirus E1 protein binds specifically DNA polymerase alpha but not replication protein A.
Bonne-Andrea C, Santucci S, Clertant P, Tillier F. Bonne-Andrea C, et al. J Virol. 1995 Apr;69(4):2341-50. doi: 10.1128/JVI.69.4.2341-2350.1995. J Virol. 1995. PMID: 7884880 Free PMC article. - Phosphorylation of human replication protein A by the DNA-dependent protein kinase is involved in the modulation of DNA replication.
Henricksen LA, Carter T, Dutta A, Wold MS. Henricksen LA, et al. Nucleic Acids Res. 1996 Aug 1;24(15):3107-12. doi: 10.1093/nar/24.15.3107. Nucleic Acids Res. 1996. PMID: 8760901 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases
Research Materials
Miscellaneous