CD28-mediated up-regulation of beta 1-integrin adhesion involves phosphatidylinositol 3-kinase - PubMed (original) (raw)
. 1996 Feb 1;156(3):883-6.
Affiliations
- PMID: 8558013
CD28-mediated up-regulation of beta 1-integrin adhesion involves phosphatidylinositol 3-kinase
T Zell et al. J Immunol. 1996.
Abstract
Cross-linking of the CD28 Ag on T cells results in increased beta 1-integrin-mediated adhesion to fibronectin. Chimeric contructs containing the CD28 cytoplasmic domain fused to the extracellular and transmembrane regions of CD2 were expressed in HL60 cells to investigate CD28-mediated regulation of adhesion. Ab cross-linking of the CD2/28 chimera resulted in increased beta 1-dependent adhesion of HL60 transfectants to fibronectin. Induced binding was completely inhibited by the phosphatidylinositol 3-kinase (PI 3-K) inhibitor wortmannin. Cross-linking of the CD2/28 chimera also induced association of the p85 subunit of PI 3-K with the CD2/28 cytoplasmic domain. In contrast, cross-linking of a CD2/28 chimera containing a tyrosine to phenylalanine substitution in the YMNM motif did not result in increased adhesion to fibronectin and did not lead to association of the chimera with PI 3-K. These results directly implicate the YMNM motif and PI 3-K in the regulation of beta 1-integrin activity by the CD28 Ag.
Similar articles
- A role for phosphatidylinositol 3-kinase in the regulation of beta 1 integrin activity by the CD2 antigen.
Shimizu Y, Mobley JL, Finkelstein LD, Chan AS. Shimizu Y, et al. J Cell Biol. 1995 Dec;131(6 Pt 2):1867-80. doi: 10.1083/jcb.131.6.1867. J Cell Biol. 1995. PMID: 8557753 Free PMC article. - Activation-dependent changes in soluble fibronectin binding and expression of beta1 integrin activation epitopes in T cells: relationship to T cell adhesion and migration.
Woods ML, Cabañas C, Shimizu Y. Woods ML, et al. Eur J Immunol. 2000 Jan;30(1):38-49. doi: 10.1002/1521-4141(200001)30:1<38::AID-IMMU38>3.0.CO;2-B. Eur J Immunol. 2000. PMID: 10602025 - Regulating integrin-mediated adhesion: one more function for PI 3-kinase?
Shimizu Y, Hunt SW 3rd. Shimizu Y, et al. Immunol Today. 1996 Dec;17(12):565-73. doi: 10.1016/s0167-5699(96)10061-x. Immunol Today. 1996. PMID: 8991288 Review. No abstract available.
Cited by
- In-depth analysis of the interplay between oncogenic mutations and NK cell-mediated cancer surveillance in solid tumors.
Pesini C, Artal L, Paúl Bernal J, Sánchez Martinez D, Pardo J, Ramírez-Labrada A. Pesini C, et al. Oncoimmunology. 2024 Jul 18;13(1):2379062. doi: 10.1080/2162402X.2024.2379062. eCollection 2024. Oncoimmunology. 2024. PMID: 39036370 Free PMC article. Review. - PI3K in T Cell Adhesion and Trafficking.
Johansen KH, Golec DP, Thomsen JH, Schwartzberg PL, Okkenhaug K. Johansen KH, et al. Front Immunol. 2021 Aug 6;12:708908. doi: 10.3389/fimmu.2021.708908. eCollection 2021. Front Immunol. 2021. PMID: 34421914 Free PMC article. Review. - CTLA-4 and PD-1 Control of T-Cell Motility and Migration: Implications for Tumor Immunotherapy.
Brunner-Weinzierl MC, Rudd CE. Brunner-Weinzierl MC, et al. Front Immunol. 2018 Nov 27;9:2737. doi: 10.3389/fimmu.2018.02737. eCollection 2018. Front Immunol. 2018. PMID: 30542345 Free PMC article. Review. - Gliotoxin Suppresses Macrophage Immune Function by Subverting Phosphatidylinositol 3,4,5-Trisphosphate Homeostasis.
Schlam D, Canton J, Carreño M, Kopinski H, Freeman SA, Grinstein S, Fairn GD. Schlam D, et al. mBio. 2016 Apr 5;7(2):e02242. doi: 10.1128/mBio.02242-15. mBio. 2016. PMID: 27048806 Free PMC article. - Mechanisms of T cell organotropism.
Fu H, Ward EJ, Marelli-Berg FM. Fu H, et al. Cell Mol Life Sci. 2016 Aug;73(16):3009-33. doi: 10.1007/s00018-016-2211-4. Epub 2016 Apr 1. Cell Mol Life Sci. 2016. PMID: 27038487 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Other Literature Sources
Research Materials
Miscellaneous