Generation and characterization of urokinase receptor-deficient mice - PubMed (original) (raw)

Generation and characterization of urokinase receptor-deficient mice

M Dewerchin et al. J Clin Invest. 1996.

Abstract

Mice homozygously deficient for the urokinase-type plasminogen activator (u-PA) receptor (u-PAR-1-) were generated by homologous recombination in D3, embryonic stem cells. The genomic sequences comprising exon 2 through 5 of the u-PAR gene were replaced by the neomycin resistance gene, resulting in inactivation of both u-PAR splice variants. The inactivated u-PAR allele was transmitted via mendelian inheritance, and fertility. Inactivation of u-PAR was confirmed by the absence of binding of rabbit anti-murine u-PAR or of an aminoterminal fragment of murine u-PA (mu-PA.1-48) to u-PAR-1- embryonic fibroblasts and macrophages. u-PAR-1- mice displayed normal lysis of a murine plasma clot injected via the jugular vein. Invasion of macrophages into the peritoneal cavity after thioglycollate stimulation was similar in u-PAR-1- and u-PAR-1- mice. u-PAR-1- peritoneal macrophages had a threefold decreased initial rate of u-PA-mediated plasminogen activation in vitro but degraded extracellular matrix proteins in vitro as efficiently as u-PAR-1- macrophages.

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References

    1. J Exp Med. 1974 Apr 1;139(4):834-50 - PubMed
    1. J Biol Chem. 1994 Oct 21;269(42):25992-8 - PubMed
    1. J Embryol Exp Morphol. 1985 Jun;87:27-45 - PubMed
    1. Biochim Biophys Acta. 1986 Dec 10;884(3):402-8 - PubMed
    1. J Biol Chem. 1987 Apr 5;262(10):4437-40 - PubMed

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