betaII protein kinase C is required for the G2/M phase transition of cell cycle - PubMed (original) (raw)
. 1996 Jun 21;271(25):15045-53.
doi: 10.1074/jbc.271.25.15045.
Affiliations
- PMID: 8663071
- DOI: 10.1074/jbc.271.25.15045
Free article
betaII protein kinase C is required for the G2/M phase transition of cell cycle
L J Thompson et al. J Biol Chem. 1996.
Free article
Abstract
Entry into mitosis requires the coordinated action of multiple mitotic protein kinases. In this report, we investigate the involvement of protein kinase C in the control of mitosis in human cells. Treatment of synchronized HL60 cells with the highly selective protein kinase C (PKC) inhibitor chelerythrine chloride leads to profound cell cycle arrest in G2 phase. The cellular effects of chelerythrine are not due to either direct or indirect inhibition of the known mitotic regulator p34(cdc2)/cyclin B kinase. Rather, several lines of evidence demonstrate that chelerythrine-mediated G2 phase arrest results from selective inhibition and degradation of betaII protein kinase C. First, chelerythrine causes dose-dependent inhibition of betaII PKC in vitro with an IC50 identical to that for G2 phase blockade in whole cells. Second, chelerythrine specifically inhibits betaII PKC-mediated lamin B phosphorylation and mitotic nuclear lamina disassembly. Third, chelerythrine leads to selective loss of betaII PKC during G2 phase in synchronized cells. Fourth, chelerythrine mediates activation-dependent degradation of PKC, indicating that betaII PKC is selectively activated during G2 phase of cell cycle. Taken together, these data demonstrate that betaII PKC activation at G2 phase is required for mitotic nuclear lamina disassembly and entry into mitosis and that betaII PKC-mediated phosphorylation of nuclear lamin B is important in these events.
Similar articles
- Identification of nuclear beta II protein kinase C as a mitotic lamin kinase.
Goss VL, Hocevar BA, Thompson LJ, Stratton CA, Burns DJ, Fields AP. Goss VL, et al. J Biol Chem. 1994 Jul 22;269(29):19074-80. J Biol Chem. 1994. PMID: 8034666 - Mapping of a molecular determinant for protein kinase C betaII isozyme function.
Gökmen-Polar Y, Fields AP. Gökmen-Polar Y, et al. J Biol Chem. 1998 Aug 7;273(32):20261-6. doi: 10.1074/jbc.273.32.20261. J Biol Chem. 1998. PMID: 9685375 - Linking protein kinase C to cell-cycle control.
Livneh E, Fishman DD. Livneh E, et al. Eur J Biochem. 1997 Aug 15;248(1):1-9. doi: 10.1111/j.1432-1033.1997.t01-4-00001.x. Eur J Biochem. 1997. PMID: 9310352 Review. - The role of protein kinase C in G1 and G2/M phases of the cell cycle (review).
Fishman DD, Segal S, Livneh E. Fishman DD, et al. Int J Oncol. 1998 Jan;12(1):181-6. doi: 10.3892/ijo.12.1.181. Int J Oncol. 1998. PMID: 9454903 Review.
Cited by
- Mitotic lamin disassembly is triggered by lipid-mediated signaling.
Mall M, Walter T, Gorjánácz M, Davidson IF, Nga Ly-Hartig TB, Ellenberg J, Mattaj IW. Mall M, et al. J Cell Biol. 2012 Sep 17;198(6):981-90. doi: 10.1083/jcb.201205103. J Cell Biol. 2012. PMID: 22986494 Free PMC article. - Visual snapshots of intracellular kinase activity at the onset of mitosis.
Dai Z, Dulyaninova NG, Kumar S, Bresnick AR, Lawrence DS. Dai Z, et al. Chem Biol. 2007 Nov;14(11):1254-60. doi: 10.1016/j.chembiol.2007.10.007. Chem Biol. 2007. PMID: 18022564 Free PMC article. - Phosphorylation of nuclear phospholipase C beta1 by extracellular signal-regulated kinase mediates the mitogenic action of insulin-like growth factor I.
Xu A, Suh PG, Marmy-Conus N, Pearson RB, Seok OY, Cocco L, Gilmour RS. Xu A, et al. Mol Cell Biol. 2001 May;21(9):2981-90. doi: 10.1128/MCB.21.9.2981-2990.2001. Mol Cell Biol. 2001. PMID: 11287604 Free PMC article. - Lipid-dependent and -independent regulation of nuclear envelope disassembly.
Domart MC, Larijani B. Domart MC, et al. J Chem Biol. 2012 Dec 18;6(1):3-5. doi: 10.1007/s12154-012-0088-8. eCollection 2012. J Chem Biol. 2012. PMID: 24348875 Free PMC article. No abstract available. - Protein kinase C isozymes as therapeutic targets for treatment of human cancers.
Fields AP, Murray NR. Fields AP, et al. Adv Enzyme Regul. 2008;48:166-78. doi: 10.1016/j.advenzreg.2007.11.014. Epub 2008 Mar 18. Adv Enzyme Regul. 2008. PMID: 18167314 Free PMC article. No abstract available.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases
Miscellaneous